PMID: 8588343Nov 1, 1995Paper

Detection of cytokine transcriptional profiles from bovine peripheral blood mononuclear cells and CD4+ lymphocytes by reverse transcriptase polymerase chain reaction

Veterinary Immunology and Immunopathology
J Covert, G Splitter

Abstract

Cytokine transcriptional profiles constitute important information about T cell mediated immunity against pathogens. We have developed a reverse transcriptase polymerase chain reaction (RT-PCR) assay to monitor gene expression of bovine T lymphocyte cytokines. Bovine cDNA was reverse transcribed from total RNA and subsequently amplified using primers designed from bovine or murine and human consensus sequences. Cytokine transcription of beta-actin, interleukins (IL) IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)alpha, TNF beta and interferon-gamma was detected from concanavalin A activated peripheral blood mononuclear cells and CD4+ purified T lymphocytes. The assay allows detection of many cytokine mRNA in a species where research has been hindered by lack of commercially available reagents and sequence information. RT-PCR analysis of lymphocyte cytokines will be invaluable for understanding the progression or resolution of disease as a consequence of lymphocyte response to specific antigens.

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Citations

Nov 9, 2000·Veterinary Immunology and Immunopathology·K TakeharaM Nakamura
Jun 8, 2001·Veterinary Immunology and Immunopathology·C Van Kampen, B A Mallard
Aug 11, 2001·Veterinary Immunology and Immunopathology·T VerfaillieB M Goddeeris
May 1, 1997·Veterinary Immunology and Immunopathology·R G KeefeJ L Stott
Jan 22, 1998·Veterinary Immunology and Immunopathology·C M DozoisI P Oswald
Feb 9, 1999·Veterinary Immunology and Immunopathology·S Giguère, J F Prescott
Feb 26, 2000·Veterinary Immunology and Immunopathology·P McWatersJ P Scheerlinck
Jun 28, 2003·Veterinary Microbiology·Satoru KonnaiMisao Onuma
Oct 11, 2008·Developmental and Comparative Immunology·Masahiro YasudaTakayuki Murakami
Jun 5, 2007·Veterinary Immunology and Immunopathology·Sachi TanakaTakahiro Yamaguchi
Feb 7, 2006·Journal of Immunological Methods·Sangeeta BudhiaBarbara A Blacklaws
Jul 1, 1997·The Journal of Veterinary Medical Science·H OkadaT J Rosol
Aug 28, 2004·The American Journal of Pathology·Raghava PotulaShilpa Buch
Nov 7, 2001·The Journal of Parasitology·J M VoyichD E Burgess
Feb 19, 2000·Biochemical and Biophysical Research Communications·J LouP R Manske

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