Detection of dairy Leuconostoc strains using the polymerase chain reaction

Letters in Applied Microbiology
L J WardG P Davey

Abstract

This paper reports the design of a Leuconostoc-specific oligonucleotide based on 16S rRNA sequence data. When this oligonucleotide was used in a polymerase chain reaction (PCR) in conjunction with an oligonucleotide to a conserved region of the 16S rRNA sequence, a Leuconostoc-specific PCR product of approximately 470 bp was produced. The use of a second oligonucleotide to a conserved region allowed the production of an approximately 350 bp product in all PCRs, acting as a positive control. The PCR procedure described was particularly useful for detecting the presence of Leuconostoc in mixed mesophilic starter cultures. The Leuconostoc-specific oligonucleotide was used also as a specific hybridization probe.

References

Dec 14, 1991·Lancet·J P Seery
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Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
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Jun 1, 1989·Applied and Environmental Microbiology·B D JarvisE A Slade

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Citations

Sep 23, 2003·Journal of Microbiological Methods·Jichan JangHongui Han
Mar 16, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J S ZhouH S Gill
Jun 1, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Seitaro GotoItaru Watanabe
Jul 13, 2004·International Journal of Food Microbiology·Hilde Marit ØstlieSiv Skeie

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