PMID: 7543546Aug 1, 1995Paper

Detection of deiminated proteins in rat skin: probing with a monospecific antibody after modification of citrulline residues

The Journal of Investigative Dermatology
T SenshuM Manabe

Abstract

We performed a systematic study on deiminated proteins present in rat epidermis. Proteins extracted from various epidermal samples were resolved by either one- or two-dimensional gel electrophoresis and Western blotted to nitrocellulose membranes. Deiminated proteins were detected by modification of citrulline residues followed by probing with an anti-modified citrulline monospecific antibody. The cornified layer of adult plantar skin gave multiple series of isoelectric variants, most of which were found to be differentially deiminated type II keratins (60 kDa, and 67 kDa or above). The whole epidermis of 5-day-old rat back skin showed isoelectric variants of 60-kDa keratin as major deiminated components, and deiminated 55-kDa keratin and deiminated filaggrin as minor spots. In addition, we found highly deiminated proteins (200-220 kDa) thought to be derived from trichohyalin. The immunoreactivity of deiminated proteins was mainly localized in the granular and cornified layers of epidermis. Co-localization of deiminated filaggrin and keratins in the granular layer suggests the possible role of protein deimination during the terminal stage of epidermal differentiation.

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Citations

Sep 18, 2003·Arthritis and Rheumatism·Erik R VossenaarLeo A B Joosten
Jul 21, 2006·The Journal of Comparative Neurology·Reinout RaijmakersBrian Werneburg
Sep 26, 2007·Annals of the New York Academy of Sciences·Akari SuzukiKazuhiko Yamamoto
Jan 30, 2010·Cellular and Molecular Life Sciences : CMLS·Marie-Claire MéchinMichel Simon
Dec 3, 2013·Annals of the Rheumatic Diseases·Nishant Dwivedi, Marko Radic
Mar 29, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nishant DwivediMarko Radic
Mar 11, 2017·Expert Opinion on Therapeutic Targets·Sindhu KoushikSriram Rajagopal
Nov 6, 2018·Scientific Reports·Saki KawashimaAkihito Ishigami
Feb 21, 2019·The ISME Journal·M Fata MoradaliMary Ellen Davey
Dec 31, 2019·Journal of Immunology Research·Mohammed AlghamdiElrashdy M Redwan
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M MizoguchiH Ogawa
Jul 2, 1999·Experimental Dermatology·K Akiyama, T Senshu
May 1, 2002·Current Opinion in Rheumatology·ZhiJie Zhou, Henri-André Ménard
Aug 1, 2007·Arthritis and Rheumatism·Holger BangGerd-R Burmester
Apr 25, 2018·Journal of Periodontal Research·S AritaJ Yamazaki
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Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Erik R VossenaarGer J M Pruijn
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May 15, 2018·Experimental Dermatology·Laura CauMichel Simon
May 21, 2008·International Journal of Cosmetic Science·M-C MéchinM Simon

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