PMID: 3281980Mar 1, 1988

Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes

Journal of Clinical Microbiology
K E OlsonB J Beaty

Abstract

A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.

Related Concepts

Dengue Fever
Immunofluorescence Assay
Nucleic Acid Hybridization Procedure
Insect Vectors
RNA Probes
Aedes albopictus
Genomic Hybridization
RNA, Viral
Tissue Culture
Culicidae

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