PMID: 16646473May 2, 2006Paper

Detection of deoxynivalenol in foods and indoor air using an array biosensor

Environmental Science & Technology
Miriam M NgundiChris Rowe Taitt

Abstract

Deoxynivalenol (DON), a mycotoxin produced by several Fusaruim species, is a worldwide contaminant of foods and feeds. Because of the potential dangers due to accidental or intentional contamination of foods with DON, there is a need to develop a rapid and highly sensitive method for easy identification and quantification of DON. In this study, we have developed and utilized a competitive immunoassay technique to detect DON in various food matrixes and indoor air samples using an array biosensor. A DON-biotin conjugate, immobilized on a NeutrAvidin-coated optical waveguide, competed with the DON in the sample for binding to fluorescently labeled DON monoclonal antibodies. To demonstrate a simple procedure amenable for on-site use, DON-spiked cornmeal, cornflakes, wheat, barley, and oats were extracted with methanol-water (3:1) and assayed without cleanup or preconcentration. The limits of detection ranged from 0.2 ng/mL in buffer to 50 ng/g in oats. The detection limit of DON spiked into an aqueous effluent from an air sampler was 4 ng/mL.

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Citations

May 28, 2008·Analytical and Bioanalytical Chemistry·Michael Seidel, Reinhard Niessner
Apr 13, 2011·Analytical and Bioanalytical Chemistry·Denis DorokhinMichel W F Nielen
Aug 1, 2011·Mycotoxin Research·Chris M Maragos
Aug 25, 2010·Journal of Agricultural and Food Chemistry·Julie MeneelyChristopher Elliott
Feb 16, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·C M Maragos, M Busman
Mar 31, 2009·International Journal of Molecular Sciences·Pietro CozziniChiara Dall'Asta
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Frances S LiglerChristopher J Myatt
Mar 14, 2013·Expert Opinion on Medical Diagnostics·Joshua BalsamAvraham Rasooly
Jul 7, 2007·Drug and Chemical Toxicology·Miroslav PohankaKamil Kuca
Jan 31, 2017·The Analyst·Miguel OssandonAvraham Rasooly
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
Jun 15, 2010·Lab on a Chip·Steven SunAvraham Rasooly
Jul 16, 2013·Chemical Society Reviews·Jeffrey KirschAleksandr Simonian
Dec 26, 2006·Journal of Food Protection·Miriam M NgundiChris R Taitt
Jan 19, 2011·Lab on a Chip·Joshua BalsamAvraham Rasooly
Dec 15, 2008·Sensors·Chris Rowe TaittFrances S Ligler
Jan 31, 2008·Chemical Reviews·Sergey M Borisov, Otto S Wolfbeis

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