PMID: 9632742Jun 20, 1998Paper

Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
O ShimadaS Suzuki

Abstract

The expression and distribution of deoxyribonuclease I (DNase I) in human duodenum, jejunum and ileum were examined by DNase I activity assay and the reverse transcriptase-polymerase chain reaction (RT-PCR), immunofluorescence, in situ hybridization, and immunocytochemical ultrastructural analyses. High levels of DNase I were detected in the cytoplasm of Paneth cells in human small intestine. A tissue homogenate fraction rich in Paneth cells showed strong DNase I-specific enzymatic activity. Immunofluorescence analysis using several specific anti-human DNase I antibodies showed very strong immunoreactivity in the cytoplasm of every Paneth cell. In situ hybridization demonstrated high levels of DNase I mRNA in Paneth cells. Immunogold electron microscopy revealed gold particles localized along the secretory pathway, with the exocrine secretory granules mostly labeled. Our findings strongly suggest that Paneth cells synthesize and secrete DNase I into the intestinal lumen.

References

May 8, 1976·Lancet·C B RodningS L Erlandsen
Feb 1, 1977·FEBS Letters·H G MannherzR Leverman
Aug 15, 1978·European Journal of Biochemistry·G Rohr, H G Mannherz
Oct 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Lacasse, L H Martin
Jul 31, 1992·Biochemical and Biophysical Research Communications·M C PeitschJ Tschopp
Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Sep 1, 1990·Journal of Electron Microscopy Technique·Y SatohK Ono
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S ShakC L Baker
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·L BryJ I Gordon
Apr 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M SawadaH Hiai
Feb 1, 1994·The Histochemical Journal·E A Schmauder-ChockM L Patchen
Oct 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H KodamaM Nishimura
Jan 1, 1995·Advances in Experimental Medicine and Biology·S S HarwigR I Lehrer
Feb 23, 1996·The Journal of Biological Chemistry·E B MallowC L Bevins
Aug 1, 1996·Gastroenterology·S F Moss, P R Holt
Jan 1, 1996·Journal of Molecular and Cellular Cardiology·M YaoP C Kiessling
Sep 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J Ouellette, M E Selsted
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O LeisF Hernández

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Citations

Aug 3, 2001·Acta histochemica·S CetinelC Hürdağ
Apr 8, 2009·The Journal of Clinical Investigation·Edward E S NieuwenhuisRichard S Blumberg
Jun 26, 2004·Cell Structure and Function·O ShimadaS Atsumi
Feb 12, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Andrea WilcksHenk J M Aarts
May 5, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G van den EedeA Wilcks
Dec 26, 2001·The Journal of Biological Chemistry·Tokiyoshi AyabeAndre J Ouellette
Jan 17, 2019·Journal, Genetic Engineering & Biotechnology·Somia S Abdel-GanySaleh A Mohamed

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