Detection of dermcidin for sweat identification by real-time RT-PCR and ELISA

Forensic Science International : Synergy
Koichi SakuradaMineo Yoshino

Abstract

We evaluated the performance of real-time RT-PCR and ELISA assays for detection of dermcidin (DCD) in sweat and body-fluid stains. DCD, a small antibiotic peptide secreted into human sweat, was detected by real-time RT-PCR in 7-day-old stains containing as small as 10 microL of sweat, and the assay showed high specificity when testing 7-day-old stains containing 30 microL of other body-fluid. ELISA using anti-human dermcidin mouse monoclonal antibody detected DCD sweat diluted up to approximately 10,000-fold and could specifically detect DCD in 10 microL of body-fluid stains. The performance of the two assays was tested during winter on samples that simulated forensic case samples: an undershirt and a sock worn for 20 h, a handkerchief used to wipe the brow several times within 12h, a cap and a cotton glove worn for 4h, and a white robe worn at intervals for 2 years. The result showed that the former assay detected DCD in all sites of the undershirt examined (armpit, back, and breast), and the latter gave a relatively high OD value in the armpit among the three sites. For the socks, although the latter assay gave very high OD values in both the center and toe of the foot sole, the former could not detect DCD in both of them. Th...Continue Reading

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Citations

Nov 26, 2010·International Journal of Legal Medicine·Xueling OuXinyao Wu
Mar 30, 2012·Journal of Innate Immunity·Birgit Schittek
Feb 6, 2014·PloS One·Peter LandgrafMichael R Kreutz
Jun 28, 2014·BMC Research Notes·Marina MikhaylovaMichael R Kreutz
Apr 14, 2012·Legal Medicine·Koichi SakuradaMineo Yoshino
Sep 24, 2011·Legal Medicine·Koichi SakuradaMineo Yoshino
Jan 21, 2015·Forensic Science International. Genetics·C HaasJ Ballantyne
Feb 21, 2014·Forensic Science International. Genetics·M van den BergeT Sijen
Jan 19, 2015·International Journal of Medical Microbiology : IJMM·Marc Burian, Birgit Schittek
Sep 22, 2016·International Forum of Allergy & Rhinology·Elizabeth E CottrillNoam A Cohen
Mar 21, 2019·Journal of Forensic Sciences·Stacey-Ann SterlingMechthild Prinz
Nov 6, 2013·The Analyst·Nunzianda FrascioneBarbara Daniel
Oct 24, 2019·Current Pharmaceutical Design·Anjali Takke, Pravin Shende
Jul 5, 2019·Forensic Science International. Genetics·Cornelius CourtsWalther Parson
Mar 6, 2021·International Journal of Legal Medicine·Qinrui YangJianhui Xie

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