Abstract
We evaluated the performance of real-time RT-PCR and ELISA assays for detection of dermcidin (DCD) in sweat and body-fluid stains. DCD, a small antibiotic peptide secreted into human sweat, was detected by real-time RT-PCR in 7-day-old stains containing as small as 10 microL of sweat, and the assay showed high specificity when testing 7-day-old stains containing 30 microL of other body-fluid. ELISA using anti-human dermcidin mouse monoclonal antibody detected DCD sweat diluted up to approximately 10,000-fold and could specifically detect DCD in 10 microL of body-fluid stains. The performance of the two assays was tested during winter on samples that simulated forensic case samples: an undershirt and a sock worn for 20 h, a handkerchief used to wipe the brow several times within 12h, a cap and a cotton glove worn for 4h, and a white robe worn at intervals for 2 years. The result showed that the former assay detected DCD in all sites of the undershirt examined (armpit, back, and breast), and the latter gave a relatively high OD value in the armpit among the three sites. For the socks, although the latter assay gave very high OD values in both the center and toe of the foot sole, the former could not detect DCD in both of them. Th...Continue Reading
References
Apr 21, 1976·Archives of Dermatological Research·W P Herrmann, J Habbig
Oct 31, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y D KimJ T Tomita
Jun 1, 1984·Analytical Biochemistry·T Marshall
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L GalloM Bernfield
Jan 1, 1993·International Journal of Legal Medicine·A KimuraT Tsuji
Nov 22, 2000·The Journal of Biological Chemistry·J HarderJ M Schroder
Nov 6, 2001·Nature Immunology·B SchittekC Garbe
Oct 16, 2002·Journal of Immunological Methods·Thomas FladHubert Kalbacher
Aug 21, 2003·Forensic Science International : Synergy·Jane Juusola, Jack Ballantyne
Sep 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dale PorterKornelia Polyak
Apr 2, 1963·Biochimica Et Biophysica Acta·M JIRKA, J MASOPUST
Sep 21, 2004·The British Journal of Dermatology·S RiegB Schittek
Jun 9, 2005·Forensic Science International : Synergy·Jane Juusola, Jack Ballantyne
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Siegbert RiegBirgit Schittek
Nov 18, 2005·Forensic Science International : Synergy·Christa NussbaumerIrina Korschineck
Dec 16, 2005·The Journal of Biological Chemistry·Daniel BaechleHubert Kalbacher
Jun 21, 2007·International Journal of Legal Medicine·Dmitry ZubakovManfred Kayser
Jul 13, 2007·The Prostate·Grant D StewartJames A Ross
Sep 18, 2007·Journal of Forensic Sciences·Jane Juusola, Jack Ballantyne
Feb 27, 2008·Journal of Forensic Sciences·Mindy SetzerJack Ballantyne
Apr 12, 2008·Current Opinion in Clinical Nutrition and Metabolic Care·Grant D StewartVickie E Baracos
Jul 3, 2008·International Journal of Legal Medicine·Dmitry ZubakovManfred Kayser
Nov 28, 2008·Legal Medicine·Koichi SakuradaMineo Yoshino
Citations
Nov 26, 2010·International Journal of Legal Medicine·Xueling OuXinyao Wu
Dec 10, 2013·Forensic Science International. Genetics·C HaasJ Ballantyne
Mar 30, 2012·Journal of Innate Immunity·Birgit Schittek
Feb 6, 2014·PloS One·Peter LandgrafMichael R Kreutz
Jun 28, 2014·BMC Research Notes·Marina MikhaylovaMichael R Kreutz
Jul 6, 2014·PloS One·Yan XuZiqin Zhao
Dec 4, 2013·Legal Medicine·Ken WatanabeKoichi Sakurada
Jul 2, 2016·Journal of Forensic Sciences·Shusaku MatsumuraShin-Ichi Kubo
Apr 14, 2012·Legal Medicine·Koichi SakuradaMineo Yoshino
Sep 24, 2011·Legal Medicine·Koichi SakuradaMineo Yoshino
Nov 23, 2015·Forensic Science International. Genetics·Margreet van den BergeTitia Sijen
Feb 7, 2012·Analytica Chimica Acta·Vitali SikirzhytskiIgor K Lednev
Jan 21, 2015·Forensic Science International. Genetics·C HaasJ Ballantyne
Jul 15, 2015·Analytical and Bioanalytical Chemistry·Akihisa IgohKoichi Sakurada
Dec 10, 2014·Forensic Science International. Genetics·Titia Sijen
Feb 21, 2014·Forensic Science International. Genetics·M van den BergeT Sijen
Jan 19, 2015·International Journal of Medical Microbiology : IJMM·Marc Burian, Birgit Schittek
Sep 22, 2016·International Forum of Allergy & Rhinology·Elizabeth E CottrillNoam A Cohen
Mar 21, 2019·Journal of Forensic Sciences·Stacey-Ann SterlingMechthild Prinz
Nov 6, 2013·The Analyst·Nunzianda FrascioneBarbara Daniel
Oct 24, 2019·Current Pharmaceutical Design·Anjali Takke, Pravin Shende
Jul 5, 2019·Forensic Science International. Genetics·Cornelius CourtsWalther Parson
Mar 6, 2021·International Journal of Legal Medicine·Qinrui YangJianhui Xie