Detection of differentially regulated subsarcolemmal calcium signals activated by vasoactive agonists in rat pulmonary artery smooth muscle cells

American Journal of Physiology. Cell Physiology
Krishna P SubediJames S K Sham

Abstract

Intracellular calcium (Ca(2+)) plays pivotal roles in distinct cellular functions through global and local signaling in various subcellular compartments, and subcellular Ca(2+) signal is the key factor for independent regulation of different cellular functions. In vascular smooth muscle cells, subsarcolemmal Ca(2+) is an important regulator of excitation-contraction coupling, and nucleoplasmic Ca(2+) is crucial for excitation-transcription coupling. However, information on Ca(2+) signals in these subcellular compartments is limited. To study the regulation of the subcellular Ca(2+) signals, genetically encoded Ca(2+) indicators (cameleon), D3cpv, targeting the plasma membrane (PM), cytoplasm, and nucleoplasm were transfected into rat pulmonary arterial smooth muscle cells (PASMCs) and Ca(2+) signals were monitored using laser scanning confocal microscopy. In situ calibration showed that the Kd for Ca(2+) of D3cpv was comparable in the cytoplasm and nucleoplasm, but it was slightly higher in the PM. Stimulation of digitonin-permeabilized cells with 1,4,5-trisphosphate (IP3) elicited a transient elevation of Ca(2+) concentration with similar amplitude and kinetics in the nucleoplasm and cytoplasm. Activation of G protein-coupled ...Continue Reading

References

Mar 1, 1992·Cell Calcium·M D Stern
Oct 27, 1995·Science·M T NelsonW J Lederer
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·N L AllbrittonT Meyer
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S ShamM Morad
Sep 1, 1993·Medical Microbiology and Immunology·S BhakdiM Palmer
Dec 6, 1996·The Journal of Biological Chemistry·M N BadmintonC M Rembold
Apr 24, 1997·Nature·M J Berridge
Jan 31, 1998·The EMBO Journal·P LippM D Bootman
Apr 7, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·G E Hardingham, H Bading
Dec 14, 1999·Journal of Microscopy·D V GordienkoT B Bolton
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·L A ShimodaJ S Sham
Feb 9, 2000·American Journal of Physiology. Cell Physiology·J H JaggarM T Nelson
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·M F LeiteM H Nathanson
Apr 30, 2003·Nature Cell Biology·Wihelma EchevarríaMichael H Nathanson
May 13, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wei-Min ZhangJames S K Sham
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Jul 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeharu NagaiAtsushi Miyawaki
May 3, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiao-Ru YangJames S K Sham
Nov 18, 2005·Cell Calcium·Phedra MariusM Fatima Leite
Mar 3, 2006·The Journal of Clinical Investigation·Xu WuDonald M Bers
May 25, 2006·Chemistry & Biology·Amy E PalmerRoger Y Tsien
Oct 13, 2006·Cell Calcium·Michael J Berridge
Apr 5, 2007·Nature Protocols·Amy E Palmer, Roger Y Tsien
Apr 11, 2007·The Journal of Biological Chemistry·Michele A RodriguesMichael H Nathanson
Jul 20, 2007·American Journal of Physiology. Cell Physiology·Beatrice A Williams, Stephen M Sims
Sep 1, 2007·The Journal of Physiology·Aleksey V ZimaLothar A Blatter
May 10, 2008·The Journal of Physiology·Anant B Parekh
May 17, 2008·Genes & Development·Johanna AndraeChrister Betsholtz
Jul 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas HendelDierk F Reiff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.