Detection of DNA damage response in nonalcoholic fatty liver disease via p53-binding protein 1 nuclear expression

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Yuko AkazawaMasahiro Nakashima

Abstract

Nonalcoholic fatty liver disease is a major liver disease that leads to cirrhosis and/or hepatocellular carcinoma in a subset of patients. The mechanism underlying disease progression is largely unknown. p53-binding protein 1 (53BP1) is a DNA damage response protein that rapidly localizes at the site of DNA double-strand breaks. In this study, we investigated nuclear 53BP1-positive foci formation as an indicator of DNA double-strand breaks in human nonalcoholic fatty liver disease liver tissues by immunofluorescence microscopy. A total of 52 liver tissue samples, including 43 nonalcoholic fatty liver disease samples and 9 controls, were studied. Our results show that the number of abnormal 53BP1-positive foci in hepatocytes (defined as three or more discrete nuclear foci and/or large foci greater than 1 μM) was significantly increased in nonalcoholic fatty liver disease patients compared to that in controls, both in nonalcoholic fatty liver (p < 0.01) and nonalcoholic steatohepatitis patients (p < 0.01). The number of large foci was significantly increased in the nonalcoholic steatohepatitis cases compared to that in the nonalcoholic fatty liver cases (p < 0.05) and correlated with increased stage of fibrosis. The number of lar...Continue Reading

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Citations

Nov 6, 2020·Drug and Chemical Toxicology·Chiagoziem A Otuechere, Ebenezer O Farombi
Dec 3, 2020·International Journal of Molecular Sciences·Maria Mercado-GómezTeresa C Delgado
Jul 13, 2021·Journal of Atherosclerosis and Thrombosis·Daiju FukudaMasataka Sata
Aug 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Siarhei A DabravolskiAlexander N Orekhov

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Methods Mentioned

BETA
biopsy
biopsies
Fluorescence
fluorescence microscopy

Software Mentioned

SAS

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