Detection of DNA hybridization on a liposome surface using ultrasound velocimetry and turbidimetry methods

Bioorganic & Medicinal Chemistry Letters
T HianikPankaj Vadgama

Abstract

19-mer oligonucleotides with oleylamine tethered at 3' and 5' terminal, respectively, were incorporated into unilamellar liposomes of dioleoylphosphatidylcholine (DOPC). Addition of complementary nucleotide resulted in hybridization with oligonucleotides located on different liposomes and caused liposome aggregation. Significant changes of sound velocimetry and turbidity were readily observed at 10 nM concentration of the complementary chain.

Citations

Sep 6, 2006·Analytical and Bioanalytical Chemistry·Katie A Edwards, Antje J Baeumner
Jun 5, 2013·Journal of Agricultural and Food Chemistry·Nao MatsushitaYoshinari Baba
Apr 1, 2005·Journal of Microencapsulation
Jul 12, 2008·Colloids and Surfaces. B, Biointerfaces·Tatsuo MaruyamaMasahiro Goto
Oct 18, 2012·The Analyst·Qingtao Liu, Ben J Boyd

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