Detection of DNA hybridizations using solid-state nanopores.

Nanotechnology
Venkat S K BalagurusamyXinsheng Sean Ling

Abstract

We report an experimental study of using DNA translocation through solid-state nanopores to detect the sequential arrangement of two double-stranded 12-mer hybridization segments on a single-stranded DNA molecule. The sample DNA is a trimer molecule formed by hybridizing three single-stranded oligonucleotides. A polystyrene bead is attached to the end of the trimer DNA, providing a mechanism in slowing down the translocation and suppressing the thermal diffusion, thereby allowing the detection of short features of DNA by standard patch-clamp electronics. The electrical signature of the translocation of a trimer molecule through a nanopore has been identified successfully in the temporal traces of ionic current. The results reported here represent the first successful attempt in using a solid-state nanopore as an ionic scanning device in resolving individual hybridization segments (or 'probes') on a DNA molecule.

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J KasianowiczD W Deamer
Jul 22, 1996·Physical Review Letters·W Sung, P J Park
Oct 8, 1999·Biophysical Journal·D K Lubensky, D R Nelson
Apr 6, 2001·Physical Review Letters·M Muthukumar
May 1, 2001·Physical Review Letters·A MellerD Branton
Jul 13, 2001·Nature·J LiJ A Golovchenko
Jul 15, 2003·Nature Materials·A J StormC Dekker
Sep 7, 2004·Biophysical Journal·Jérôme MathéAmit Meller
Mar 30, 2005·Nano Letters·Miloslav KarhanekChris D Webb
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A J StormC Dekker
Aug 11, 2005·Physical Review Letters·Nolan C Harris, Ching-Hwa Kiang
Oct 11, 2008·Nature Biotechnology·Daniel BrantonJeffery A Schloss
May 8, 2009·Nanotechnology·Hongbo Peng, Xinsheng Sean Ling
Jun 6, 2009·Analytical Chemistry·Bo ZhangHyunae Lee
Jun 3, 2010·Nano Letters·Lorenz J SteinbockUlrich F Keyser

❮ Previous
Next ❯

Citations

May 27, 2011·Analytical Chemistry·Thomas P NiedringhausAnnelise E Barron
Oct 25, 2011·Analytical Chemistry·Christopher Batchelor-McAuleyRichard G Compton
Aug 31, 2012·Journal of the American Chemical Society·Leyla EsfandiariJacob J Schmidt
Sep 1, 2014·The Review of Scientific Instruments·Stanislav PolonskyJohn A Ott
Mar 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Benjamin A EvansAlison E Deatsch
Jan 23, 2015·Frontiers in Genetics·Yue WangZhimin Wang
Jul 11, 2018·Lab on a Chip·Bonhye KooHarold G Monbouquette
Oct 13, 2012·Nanotechnology·Valentina MussiUgo Valbusa
Aug 22, 2013·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Daniel Y Ling, Xinsheng Sean Ling
Oct 23, 2019·Scientific Reports·Angus McMullenDerek Stein
Feb 26, 2011·Genome Biology·John F Thompson, Patrice M Milos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.