Detection of DNA mutations associated with mitochondrial diseases by Agilent 2100 bioanalyzer

Clinica Chimica Acta; International Journal of Clinical Chemistry
Ching-You LuYau-Huei Wei

Abstract

Molecular analysis of mitochondrial DNA (mtDNA) has provided a final diagnosis for many of the mitochondrial diseases. We evaluated the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) to determine whether the system could replace the conventional restriction fragment length polymorphism (RFLP) analysis by the agarose gel electrophoresis for the detection of the mtDNA mutation. Three members of a family with MELAS syndrome and four members of a family with MERRF syndrome were recruited for this study. After PCR and restriction enzyme digestion, DNA fragments were separated on the Agilent 2100 bioanalyzer in conjunction with the DNA 500 and DNA 1000 Labchip kits and by electrophoresis on precast 3% agarose gels. The data generated by the DNA 500 and DNA 1000 assays using the Agilent 2100 bioanalyzer showed a lower percentage error and a better reproducibility as compared to those obtained by the conventional method. Based on the performance of the bioanalyzer, we suggest that this novel Labchip is adequate to replace the current RFLP analysis by the agarose gel electrophoresis for mtDNA mutation detection.

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Citations

Aug 9, 2005·Applied and Environmental Microbiology·Hikaru SuenagaTakahiro Kanagawa
Jan 5, 2007·Journal of Clinical Microbiology·Lucy J HathawayKathrin Mühlemann
Jan 8, 2016·Human Molecular Genetics·Ian J WilsonPatrick F Chinnery
May 6, 2004·Annals of the New York Academy of Sciences·Chun-Yi LiuYau-Huei Wei
Apr 14, 2016·BioTechniques·Jessica DaviesJames Hadfield
Feb 18, 2010·Lab on a Chip·Hun Joo LeeSoo Suk Lee
Feb 25, 2018·Applied and Environmental Microbiology·Xia SuJing Fang
Apr 19, 2005·Expert Review of Molecular Diagnostics·Maurizio FerrariBarbara Foglieni

Related Concepts

Teens
DNA Mutational Analysis
DNA, Mitochondrial
Electrophoresis
Personal Computers
Genealogical Tree
Restriction Fragment Length Polymorphism
Melas Syndrome
Merrf Syndrome
Muscle Weakness

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