Detection of drug-induced apoptosis and necrosis in human cervical carcinoma cells using 1H NMR spectroscopy

Cell Death and Differentiation
T BezabehI C Smith

Abstract

Apoptosis and necrosis need to be differentiated in order to distinguish drug-induced cell death from spontaneous cell death due to hypoxia. The ability to differentiate between these two modes of cell death, especially at an early stage in the process, could have a significant impact on accessing the outcome of anticancer drug therapy in the clinic. Nuclear magnetic resonance spectroscopy was used to distinguish apoptosis from necrosis in human cervical carcinoma (HeLa) cells. Apoptosis was induced by treatment with the topoisomerase II inhibitor etoposide, whereas necrosis was induced by the use of ethacrynic acid or cytochalasin B. We found that the intensity of the methylene resonance increases significantly as early as 6 h after the onset of apoptosis, but that no such changes occur during necrosis. The spectral intensity ratio of the methylene to methyl resonances also shows a high correlation with the percentage of apoptotic cells in the sample (r2=0.965, P<0.003).

References

Jan 1, 1974·Journal of Supramolecular Structure·S Lin, J A Spudich
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Jan 1, 1995·Methods in Cell Biology·A J McGahonD R Green
Nov 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P R Walker, M Sikorska
Apr 15, 1994·Cancer·J F KerrB V Harmon
Jan 17, 1996·International Journal of Cancer. Journal International Du Cancer·E J DelikatnyC E Mountford
Aug 1, 1997·Trends in Biochemical Sciences·D W Nicholson, N A Thornberry
Jun 19, 1998·Biochimica Et Biophysica Acta·L BoginH Degani
Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K R Hande
Apr 14, 1999·Cell Death and Differentiation·V A FadokP M Henson
Mar 18, 2000·The Journal of Biological Chemistry·D K PerryY A Hannun
Aug 1, 1997·Cell Death and Differentiation·P Nicotera, M Leist

❮ Previous
Next ❯

Citations

Sep 23, 2003·Biochimica Et Biophysica Acta·Nalayini SathasivamEdward J Delikatny
Oct 7, 2004·Journal of the National Cancer Institute·Magnus LindskogPer Kogner
Mar 20, 2004·Expert Review of Cardiovascular Therapy·María A FortuñoJavier Díez
Nov 5, 2004·Experimental Lung Research·Aiying DuBaoxiang Zhao
Jul 14, 2010·Transplantation Proceedings·X NiuG P Jeffrey
Apr 14, 2010·European Journal of Pharmacology·Zhicong HeLaurence P G Wakelin
Aug 21, 2007·Biochimica Et Biophysica Acta·Matthew MilkevitchE James Delikatny
Jun 21, 2005·Annals of the New York Academy of Sciences·Chwen-Ming ShihChien-Tsu Chen
Jun 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jonathan E SchmitzKevin M Brindle
May 4, 2011·NMR in Biomedicine·E James DelikatnyHarish Poptani
Apr 16, 2003·Biochemical Pharmacology·Fabrizia BrisdelliArgante Bozzi
Jan 23, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gunjan SharmaBiplob Koch
Jun 18, 2017·Apoptosis : an International Journal on Programmed Cell Death·Filipe ElvasLeonie Wyffels
Jun 24, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Sujay MukhopadhyayDaya Shankar Pandey
Mar 24, 2018·International Journal of Cancer. Journal International Du Cancer·Efthymia PapaevangelouYuen-Li Chung
Apr 10, 2018·AJR. American Journal of Roentgenology·Yudai NakaiKiyoshi Hasegawa
Nov 30, 2005·Journal of Neuro-oncology·Yunfeng ZhaoQingzhong Kong
Nov 22, 2005·Drug and Alcohol Review·Marianne E JaunceyLouisa J Degenhardt
Apr 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·María A FortuñoJavier Díez
Dec 7, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Rajendra Prasad PaitandiDaya Shankar Pandey
Feb 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Somayeh Handali, Mohsen Rezaei
Apr 8, 2021·Microbiology·Leah Isobella Rankine-WilsonYossef Av-Gay
Sep 29, 2017·Inorganic Chemistry·Rajendra Prasad PaitandiDaya Shankar Pandey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.