Abstract
Epstein-Barr virus is the first human oncogenic virus associated with a broad range of different malignant diseases but its role in non-Hodgkin lymphomas (NHL) development still needs to be fully understood. High expression levels of EBV major genes are found in NHL tumor cells and free viral DNA circulates in the plasma of such individuals. In the current study we detected EBV DNA levels in plasma samples from NHL patients in order to validate its significance as a laboratory marker for disease monitoring. We investigated a cohort of 52 patients diagnosed with NHL in The University Hospital "St. Marina" Varna, Bulgaria. Viral DNA was extracted from single plasma samples using Kit Ribo Virus (Sacace Biotechnologies S.r.l., Como, Italy) and amplified with EBV Real-TM Quant (Sacace Biotechnologies S.r.l., Como, Italy). Plasma samples of the same patients were tested for presence of EBV VCA IgM/IgG antibodies with indirect ELISA tests (Euroimmun, Luebeck, Germany). We found 15.4% (95% CI 6.9-28.1%, n = 8) of the samples from NHL patients to be positive in quantitative PCR (range 674-221,333 copies/ml). The diffuse large B cell lymphomas and peripheral T cell lymphomas were most often associated (although not statistically signific...Continue Reading
References
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W HenleS E Straus
Aug 17, 2000·The New England Journal of Medicine·J I Cohen
Apr 3, 2001·Journal of Clinical Microbiology·S J StevensJ M Middeldorp
Mar 20, 2004·Blood·Wing-Yan AuYok-Lam Kwong
Oct 28, 2004·The Journal of Molecular Diagnostics : JMD·Julie L RyanMargaret L Gulley
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·C D Shannon-LoweH-J Delecluse
Apr 29, 2006·Acta Oncologica·Karin Ekström-Smedby
Aug 9, 2006·Diagnostic Pathology·Rocío HassanIlana R Zalcberg
Nov 23, 2006·São Paulo Medical Journal = Revista Paulista De Medicina·Juliane Garcez MusacchioNelson Spector
Mar 6, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Eric A Engels
Jun 17, 2008·The Journal of Molecular Diagnostics : JMD·Margaret L Gulley, Weihua Tang
Jul 22, 2010·Blood·Kimberly A BertrandFrancine Laden
Feb 7, 2013·Blood·Jennifer A KanakryRichard F Ambinder
May 17, 2014·International Journal of Cancer. Journal International Du Cancer·Lauren R TerasSusan M Gapstur
Dec 22, 2015·The Lancet. Haematology·Seok Jin KimUNKNOWN Asia Lymphoma Study Group
Jan 9, 2016·Blood·Jennifer A KanakryAlexandra Valsamakis
Oct 1, 2016·Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology·Mahua SinhaJayshree R Subramanyam
Mar 18, 2017·Leukemia & Lymphoma·Yi ChenJianda Hu
Dec 2, 2017·Oncotarget·Yu Ri KimJin Seok Kim
Jan 10, 2018·BMC Public Health·Jeffrey R BrookUNKNOWN CANUE – The Canadian Urban Environmental Health Research Consortium
Mar 10, 2018·OncoTargets and Therapy·Qian FeiXia He