Abstract
Sixty-one serum specimens from 22 patients with clinically diagnosed amoebic liver abscess (ALA), 10 hospitalized patients with a variety of diseases other than amoebiasis, 12 normal healthy controls, and 17 subjects from an amoebiasis-endemic area were assayed by enzyme-linked immunosorbent assay (ELISA). The plasma membrane fraction of axenic cultures of Entamoeba histolytica HK9 separated from other subcellular fractions by differential centrifugation was used as the antigen to detect specific immunoglobulin G (IgG) and IgM antibodies. Using a single serum dilution of 1/100 and optical densities at 492 nm of 0.200 and 0.250 as the cutoff values for the IgM and IgG ELISAs, their respective sensitivities in 22 ALA patients were 91% (20 of 22) and 95% (21 of 22). In 22 patients (10 hospitalized and 12 normal healthy controls), the specificities of the IgM and IgG ELISAs were 95% (21 of 22) and 91% (20 of 22), respectively. All five asymptomatic carriers of pathogenic E. histolytica were seropositive by the IgG ELISA and the amoebic gel diffusion test (AGDT). The AGDT was positive for three of six culture-negative controls, while the IgG ELISA was positive for all six. For six asymptomatic carriers of nonpathogenic zymodemes, th...Continue Reading
References
Dec 1, 1979·Journal of Clinical Microbiology·J Yang, M T Kennedy
Feb 8, 1989·Journal of Immunological Methods·A MohimenS Mehra
Mar 30, 1985·Lancet·T F JacksonA E Simjee
Mar 1, 1986·Reviews of Infectious Diseases·J I Ravdin
Jul 26, 1986·Lancet·A K GokaM J Farthing
Aug 1, 1986·The Journal of protozoology·H M MathewsG S Visvesvara
Aug 1, 1987·The Journal of Infectious Diseases·B E TorianW E Stamm
Oct 1, 1987·Infection and Immunity·W A PetriJ I Ravdin
Nov 1, 1987·The Journal of Infectious Diseases·I MezaM Rojkind
Jan 1, 1988·The American Journal of Tropical Medicine and Hygiene·M P Joyce, J I Ravdin
Nov 1, 1988·The American Journal of Tropical Medicine and Hygiene·B M GandhiB N Tandon
Sep 25, 1965·Lancet·S J PowellR Elsdon-Dew
Jan 1, 1968·Transactions of the Royal Society of Tropical Medicine and Hygiene·G L Robinson
May 1, 1971·The American Journal of Tropical Medicine and Hygiene·I M Krupp, S J Powell
Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·T F JacksonA E Simjee
Jul 1, 1983·The American Journal of Tropical Medicine and Hygiene·J Knobloch, E Mannweiler
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·T F JacksonD J Pudifin
May 1, 1980·The American Journal of Tropical Medicine and Hygiene·H J BosB F Oostburg
Nov 1, 1980·The Journal of Infectious Diseases·Y Naot, J S Remington
Jan 1, 1981·Journal of Immunoassay·J D ConradieR Kirk
Citations
Mar 22, 2013·BMC Infectious Diseases·Tan Zi NingLim Boon Huat
Jul 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S L Reed
Apr 16, 2002·Tropical Medicine & International Health : TM & IH·Mohamed D Abd-Alla, Jonathan I Ravdin
Jun 2, 2000·Journal of Clinical Microbiology·M D Abd-AllaJ I Ravdin
Oct 1, 1992·Clinical Microbiology Reviews·D A Bruckner
Jun 1, 1993·Journal of Clinical Microbiology·B M FloresB E Torian