Detection of enteric viruses, Giardia and Cryptosporidium in two different types of drinking water treatment facilities

Water Research
M A AliS E El-Hawaary

Abstract

In this study, two types of drinking water treatment facilities (two conventional drinking water treatment plants (DWTPs) and two compact units (Cus)) were compared referring to their production capacity. Water samples were collected from three main points: (a) different water treatment steps (b) washings of sand filters and (c) distribution system at different distances from the water treatment plants. Both viruses and protozoa were concentrated from each water sample by adsorption and accumulation on the same nitrocellulose membrane filters (0.45 microm pore size). Enteroviruses were detected by plaque infectivity assay in BGM cells and HAV, HEV and Norovirus were detected by RT-PCR. Giardia and Cryptosporidium were detected by conventional staining methods and PCR. The results revealed that enterovirus load at the intake ranged between 10-15 PFU/L for the two compact units and between 4.5 and 75 PFU/L for the two conventional DWTPs. The virus load in distribution system of the first type DWTPs at 1 km from the plant was the same as that of the intake. Viruses in the other type of treatment plants CUs at 1, 5 and 7 km, were much reduced. Investigation of raw water sediments of the two DWTPs showed enterovirus counts between 1...Continue Reading

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Citations

Mar 10, 2012·Parasitology Research·Naglaa Saad M El-GebalyMaha Abu-Zekry
Dec 16, 2006·Environmental Monitoring and Assessment·Jian Guo Jiang, Yun-Fen Shen
Sep 17, 2011·International Journal of Hygiene and Environmental Health·Ibrahim Ahmed HamzaMichael Wilhelm
Aug 20, 2015·Applied Microbiology and Biotechnology·Imen OuardaniJesús L Romalde
Apr 26, 2016·The Science of the Total Environment·C Joon ChuahAlan D Ziegler
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Sep 14, 2016·Biomédica : revista del Instituto Nacional de Salud·Dioselina PeláezGerardo Nava
Sep 9, 2020·PLoS Neglected Tropical Diseases·Retina ShresthaBhanu Bhakta Neupane
Feb 25, 2017·Food and Environmental Virology·Hazem HamzaIbrahim Ahmed Hamza
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Mar 4, 2020·Acta Parasitologica·Naglaa Fathi Abd El-LatifAhmed Hamdi Mohamed Abd El-Malek
Jan 28, 2021·Food and Environmental Virology·Nicole S UpfoldCaroline Knox
Jan 20, 2019·Journal of Virological Methods·Ibrahim Ahmed Hamza, Kyle Bibby
Oct 1, 2020·Food and Waterborne Parasitology·Joyce SiwilaChishimba Mubanga
Sep 1, 2017·The Science of the Total Environment·Hazem Hamza, Ibrahim Ahmed Hamza

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