Detection of environmental estrogenicity using transgenic medaka hatchlings (Oryzias latipes) expressing the GFP-tagged choriogenin L gene

Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Md A SalamShinji Hayashi

Abstract

The discharge of environmental estrogenic substances into the environment has an adverse effect on human and wildlife, especially aquatic organisms. Therefore, a simple, practical and sensitive method of detecting environmental estrogenicity is required. Previously, we established a transgenic medaka (Oryzias latipes) strain harboring choriogenin L (ChgL) tagged with green fluorescence protein (GFP), which is expressed in the liver in response to estrogen (E2). This strain of medaka could be a very useful tool in detecting aquatic estrogenicity. The appropriate conditions for analysis of estrogenicity were determined at various E2 concentrations, exposure periods and the developmental stages of medaka hatchlings. Furthermore, the relationship between E2 concentrations and GFP fluorescence intensity was investigated. It was found that fluorescence intensity of GFP depends largely on E2 concentration, exposure time and developmental stage. Hatchling at 4-day post-hatch (DPH) showed optimum conditions for exposure to E2 with optimum GFP intensity at 9 DPH. Additionally, the exposure period was optimized so that exposure from 4 DPH for 5 days showed a significant change in GFP intensity. E2 concentrations of 0, 12.5, 25, 50 and 100...Continue Reading

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Apr 19, 2012·Environmental Health Perspectives·Okhyun LeeTetsuhiro Kudoh
Mar 23, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hairong XuXiangming Li
Apr 28, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jae-Sung RheeJae-Seong Lee
Sep 10, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Petra SpirhanzlovaAndrew J Tindall
Nov 15, 2014·Critical Reviews in Toxicology·Okhyun LeeCharles R Tyler
Jan 11, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kar Lai PoonTom J Carney

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