Detection of epidermal growth factor receptor T790M mutation by allele-specific loop mediated isothermal amplification.

Journal of Carcinogenesis
Srividya ArjunaAnirban Chakraborty

Abstract

Targeted therapy using specific inhibitors against tyrosine kinases (TKs) is a paradigm in non-small-cell lung cancer management. However, the success of TK inhibitor (TKI) therapy depends on certain activating or acquired mutations, which render sensitivity or resistance to TKIs in the patients. The acquisition of epidermal growth factor receptor (EGFR) T790M point mutation is the most common mechanism of resistance to TKI in non-small cell lung cancer. A number of molecular strategies are now available for molecular testing of non-small cell lung cancers. However, almost all of them are cost-intensive and laborious and require high-end advanced equipment. Thus, assays that are rapid, simple, and cost-effective, yet sensitive, are most ideal in clinical settings for screening such therapeutically relevant mutations. Allele-specific loop-mediated isothermal amplification assay (AS-LAMP), which is a variant of the original LAMP assay, is a promising diagnostic technique for screening single-nucleotide polymorphisms. Using commercially available plasmid constructs as template DNA, AS-LAMP assay for EGFR T790M mutation was optimized with six different sets of reaction mixture containing varying concentrations of buffer and primers...Continue Reading

References

Sep 13, 2013·Methods in Molecular Biology·Hiroka AonumaHirotaka Kanuka
Apr 17, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Helena A YuGregory J Riely
Aug 10, 2019·Molecular Diagnosis & Therapy·Arjuna SrividyaGunimala Chakraborty

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BETA
PCR
electrophoresis
genotyping
biopsy

Software Mentioned

QuantLife
QuantaLife

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