PMID: 6143252Jan 1, 1984

Detection of epsilon(gamma-glutamyl) lysine

Molecular and Cellular Biochemistry
M Griffin, J Wilson

Abstract

Detection of epsilon(gamma-glutamyl) lysine crosslinks is not only necessary for establishing the importance of the dipeptide as a post-translational modification of proteins, but provides information as to the importance of the transglutaminase enzyme in a biological system. The crosslink may be detected using both indirect and direct methodology. Indirect methods for its detection include measurement of 'masked lysines' within a protein, detection of polymer formation by gel-electrophoresis and the inhibition of crosslinking by the incorporation of small molecular weight amines into the substrate protein. Direct methods for the detection of epsilon(gamma-glutamyl) lysine require the actual isolation of the dipeptide following its release from the sample protein by exhaustive proteolytic digestion. Separation of the dipeptide from other components of the digest may be achieved by either ion-exchange chromatography or gel filtration and its qualitative identification achieved by techniques such as paper-electrophoresis or thin layer chromatography. Quantitative estimation of epsilon(gamma-glutamyl) lysine normally involves its further separation by ion-exchange chromatography and its post-column detection following derivatisati...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L LorandJ Bruner-Lorand
Sep 9, 1977·Biochemical and Biophysical Research Communications·P J BirckbichlerM K Patterson
Sep 1, 1979·The Journal of Clinical Investigation·D F Mosher, P E Schad
Sep 14, 1978·Biochemical and Biophysical Research Communications·P J BirckbichlerM K Patterson
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A Dutton, S J Singer
Feb 26, 1979·Biochimica Et Biophysica Acta·S Matacić, A G Loewy
Jan 1, 1979·Archives of Biochemistry and Biophysics·I CohenJ Bruner-Lorand
Nov 15, 1975·Experientia·A SoriaC Boulard
Jul 1, 1976·Analytical Chemistry·E BayerR Uhmann
Jan 2, 1973·Biochimica Et Biophysica Acta·P J BirckbichlerA G Loewy
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H G Williams-AshmanL Lorand
Dec 8, 1972·Annals of the New York Academy of Sciences·L LorandA Gray
Dec 8, 1972·Annals of the New York Academy of Sciences·S I Chung
Dec 8, 1972·Annals of the New York Academy of Sciences·J J PisanoJ S Finlayson
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu
Nov 17, 1970·Biochimica Et Biophysica Acta·R S AsquithK L Gardner
Jan 26, 1972·Biochimica Et Biophysica Acta·H W Harding, G E Rogers
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J J PisanoY Nagai
Feb 26, 1968·Biochemical and Biophysical Research Communications·S Matacić, A G Loewy
Apr 19, 1968·Biochemical and Biophysical Research Communications·L LorandS Tokura
Feb 1, 1969·Analytical Biochemistry·M L Kakade, I E Liener
Sep 22, 1966·Biochemical and Biophysical Research Communications·S Matacić, A G Loewy
Feb 21, 1967·Biochimica Et Biophysica Acta·K R Woods, K T Wang
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L LorandN S Rafferty
Oct 13, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·M GriffinT Palmer
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·O J BjerrumL Lorand
Dec 1, 1960·The Biochemical Journal·K J CARPENTER
Apr 23, 1962·Biochimica Et Biophysica Acta·K A WALSH, J R BROWN
Jul 1, 1963·Biochemistry·M L KORNGUTHH WAELSCH
Jan 1, 1972·Methods in Enzymology·W R Gray

Citations

Jun 2, 2005·Biomaterials·David Y S ChauM Griffin
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, Kapil Mehta
May 11, 2002·Breast Cancer Research and Treatment·Jack S K ChenKapil Mehta
Jun 15, 1997·The Journal of Clinical Investigation·T S JohnsonA Meguid El Nahas
Nov 16, 2007·Journal of the American Society of Nephrology : JASN·Tim S JohnsonM Griffin
May 10, 1991·International Journal of Cancer. Journal International Du Cancer·S el AlaouiG Quash
Jun 1, 1986·Journal of Neurochemistry·C C Miller, B H Anderton

Related Concepts

N-epsilon-(gamma-L-glutamyl)lysine
Acyltransferase
High Pressure Liquid Chromatography Procedure
Ion-Exchange Chromatography Procedure
Dipeptides
Red Cell Ghost
Antithrombin I
Hydrolysis
Peptide Hydrolases
Transglutaminases

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.