Detection of exon polymorphisms in the human lactoferrin gene

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Li-Herng Eric LiuChristina Teng

Abstract

We previously demonstrated that lactoferrin gene polymorphisms occur in cancer cells of patients with leukemia and breast cancer. In this study, we established a non-radioactive polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, one of the most sensitive and simplest methods to detect polymorphisms and mutations of the human lactoferrin gene. We optimized the PCR conditions for nine different DNA templates and 16 pairs of exon primers for SSCP analysis. The DNA templates used in the analyses were prepared from a cosmid clone (CT6-1) that contains the human lactoferrin gene, human placental tissue, leukocytes from 10 normal volunteers, leukemic cells of two patients, and previously established three breast and two leukemic cell lines. With the appropriate exon-primer sets, PCR products from exon I to exon 16 of the lactoferrin gene were generated from the DNA templates and analyzed by SSCP. Compared with the homogenous cloned DNA, lactoferrin gene polymorphisms were detected within exons 2, 5, 7, 9, 13, 14, and 15 of the normal placental and leukocyte DNA. In addition, abnormal migration patterns of the lactoferrin gene in cancer cells were detected in exons 4, 5, 13, 14, and 15. The PCR-SSCP ...Continue Reading

References

Jun 1, 1992·Genetic Analysis, Techniques and Applications·K Hayashi
May 1, 1992·Archives of Disease in Childhood·L SánchezJ H Brock
Jul 25, 1991·Nucleic Acids Research·L SpinardiC Theillet
Nov 25, 1991·Nucleic Acids Research·M IizukaT Sekiya
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Jan 1, 1985·Human Genetics·D N CooperJ Schmidtke
Jan 1, 1988·Advances in Genetics·B H BowmanG S Adrian
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I ParkM M Zakin
Sep 1, 1995·Immunology Today·J Brock
Jan 1, 1995·Annual Review of Nutrition·B Lönnerdal, S Iyer
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·K Hayashi
Jun 17, 1999·International Journal of Cancer. Journal International Du Cancer·T ShishikuraA Nakagawara
Aug 24, 1999·Cancer Letters·W ThangniponK Hayashi
Jul 1, 1996·Journal of Mammary Gland Biology and Neoplasia·J H NuijensF L Schanbacher
Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christina T Teng

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Citations

Jan 30, 2004·Regulatory Toxicology and Pharmacology : RTP·Steve TaylorMary Murphy
Mar 8, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jamal A MohamedPablo C Okhuysen
Mar 12, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Christina T Teng
Nov 16, 2004·International Journal of Cancer. Journal International Du Cancer·Monique BenaïssaAnnick Pierce
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Hironobu IijimaMasatomo Mori
Nov 20, 2014·Nutrition Reviews·Yunlei ZhangLigia R Rodrigues
May 21, 2010·Journal of Applied Oral Science : Revista FOB·Luiza Foltran AzevedoPaula Cristina Trevilatto
Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jeremy H Brock

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