Detection of fecal coliforms in water by using [14C]mannitol.

Applied and Environmental Microbiology
D J Reasoner, E E Geldreich

Abstract

Interest in rapid bacterial detection methods for sanitary indicator bacteria in water prompted a study of the use of [U-14C]mannitol to detect fecal coliforms (FC). A simple method which used m-FC broth, membrane filtration, and two-temperature incubation (35 degrees C for 2 h followed by 44.5 degrees C for 2.5 h) was developed. [U-14C]mannitol was added to the medium, and the temperature was raised to 44.5 degrees C after 2 h at 35 degrees C. 14CO2 was collected as Ba14CO3 and assayed by liquid scintillation spectroscopy. Correlations were examined between FC cell numbers at the start of incubation (standard 24-h FC test) and Ba14CO3 counts per minute after 4.5 h. Results indicated that FC numbers ranging from 1 x 10(1) to 2.1 x 10(5) cells could be detected in 4.5 h. Within-sample reproducibility at all cell concentrations was good, but sample-to-sample reproducibility was variable. Comparisons between m-FC broth and m-FC broth modified by substituting D-mannitol for lactose indicated that the standard m-FC broth was the better test medium. Results from experiments in which dimethyl sulfoxide was used to increase permeability of FC to [U-14C]mannitol indicated no increase in 14CO2 production due to dimethyl sulfoxide. Detect...Continue Reading

References

Aug 1, 1979·Applied and Environmental Microbiology·D J ReasonerE E Geldreich
Aug 1, 1974·Applied Microbiology·U Bachrach, Z Bachrach
Apr 1, 1984·Journal of Bacteriology·H RosenbergP A Jacomb
Jun 1, 1982·Applied and Environmental Microbiology·P C Feng, P A Hartman
Nov 1, 1980·Applied and Environmental Microbiology·M W LeChevallierT M Evans
Nov 1, 1956·American Journal of Public Health and the Nation's Health·H C GURNEYG V LEVIN

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Citations

Dec 1, 1998·Journal of Applied Microbiology·G A McFetersS C Broadaway

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