Detection of feline upper respiratory tract disease pathogens using a commercially available real-time PCR test

The Veterinary Journal
Annette L LitsterC M Leutenegger

Abstract

Feline herpesvirus (FHV-1), feline calicivirus (FCV), Bordetella bronchiseptica (Bb), Chlamydia felis (Cf) and Mycoplasma felis (Mf) are common infectious agents identified in cats with upper respiratory tract disease (URTD). Each of these agents can either act as primary pathogens or cause subclinical infections, and pathogen identification can be used to prevent disease transmission in shelters, or to manage individual cats with recurrent URTD. The aim of this study was to compare pathogen detection rates using real-time PCR testing and virus isolation (VI) or bacterial culture in conjunctival, nasal and oropharyngeal swabs from 18 shelter-housed cats with clinical URTD. Co-infections were common; FHV-1 was most prevalent and Cf and FCV were least prevalent. Agents detected by PCR were FCV 2/18 (11%), FHV-1 17/18 (94%), Bb 8/18 (44%) and Mf 15/18 (83%). Agents detected by VI and bacterial culture were FCV 1/18 (6%), FHV-1 12/18 (67%), Bb 8/18 (44%) and Mf 12/18 (67%). Agreement between PCR results and the other two methods was: FHV-1, 57.4%; FCV, 98.1%; Bb, 75.0%; Mf, 60.0%. Discordancies included PCR-positive, VI-negative (FCV, n = 1/54, 1.9%; FHV-1, n = 23/54, 42.6%), PCR-positive, culture-negative (Bb, n = 6/36, 16.7%; Mf,...Continue Reading

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Citations

May 11, 2016·The Veterinary Journal·Michael P ReichelFraser I Hill
Feb 29, 2016·JFMS Open Reports·Sara RaviciniMichael R Lappin
Jan 22, 2019·Journal of Feline Medicine and Surgery·Santiago Peralta, Patrick C Carney
Jun 28, 2019·Journal of Feline Medicine and Surgery·Lucy KopecnyLynelle R Johnson
Nov 23, 2018·Journal of Feline Medicine and Surgery·Dalton NguyenMichael P Ward
Oct 17, 2020·Journal of the American Veterinary Medical Association·Robyn A JaynesMelissa A Kennedy

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