Detection of female lower genital tract dysplasia using orally administered 5-aminolevulinic acid induced protoporphyrin IX: a preliminary study

Gynecologic Oncology
Linda R DuskaTayyaba Hasan

Abstract

Previous studies have suggested that 5-aminolevulinic acid (ALA) may be used topically on the cervix to allow optical detection of cervical dysplasia, based on the fluorescence of protoporphyrin IX (PpIX) synthesized in situ from ALA. However, the uniformity of distribution of topically applied PpIX and the sensitivity and specificity of detection are not optimal. The current study was undertaken to demonstrate the feasibility of administering ALA by mouth (po) with the hypothesis that systemic administration might provide a more reliable diagnostic tool. Oral ALA was administered to 14 patients with abnormal Pap smears in a dose- and time-intensity design. Institutional review board approval was obtained. A starting dose of 10 mg/kg of po ALA was administered and colposcopy was performed in 3 patients at 1 h, 3 patients at 2 h, 6 patients at 3 h, and 2 patients at 4 h. The study was written with the intent to increase the dose in 10 mg/kg increments if fluorescence was not detected; however, fluorescence was detected at the first dose level. Liver function tests were checked pre and post ALA and follow-up telephone calls were made regarding possible side effects. Both white and blue light colposcopy examinations were performed...Continue Reading

References

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Citations

Apr 1, 2010·Chemical Reviews·Jonathan P CelliTayyaba Hasan
Apr 11, 2009·Gynécologie, obstétrique & fertilité·P CollinetS Mordon
Jun 1, 2007·Photodiagnosis and Photodynamic Therapy·Pierre CollinetSerge Mordon
Oct 18, 2014·Medicina·Rasa VansevičiūtėSimona Letautienė

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