Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode.

International Journal of Nanomedicine
Guangxian ZhongJianhua Lin

Abstract

In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH(3))(6)](3+), as the electrochemical indicator. The effect of different frequencies on the real surface area of the nanostructure gold electrode obtained by repetitive square-wave oxidation reduction cycle was investigated. At the optimal frequency of 8000 Hz, the real surface of the developed nanostructure gold electrode was about 42.5 times compared with that of the bare planar gold electrode. The capture probe DNA was immobilized on the nanostructure gold electrode and hybridized with target DNA. Electrochemical signals of hexaamine-ruthenium III bound to the anionic phosphate of DNA strands via electrostatic interactions were measured by chronocoulometry before and after hybridization. The increase of the charges of hexaamine-ruthenium III was observed upon hybridization of the probe with target DNA. Results indicate that this DNA biosensor could detect the femtomole (fM) concentration of the DNA target quantitatively in the range of 50 fM to 250 fM;...Continue Reading

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Citations

Dec 11, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yong DingYong Zhang
Jul 15, 2015·Journal of Electroanalytical Chemistry·Wanda I PérezEnrique Meléndez
Jul 5, 2013·Applied Biochemistry and Biotechnology·Sandip Kumar DashAshok Kumar
Dec 29, 2020·International Journal of Nanomedicine·Dakai ZhouThangavel Lakshmipriya
Jun 4, 2021·Biotechnology and Applied Biochemistry·Jie LeiLiang-Zhou Xu

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Methods Mentioned

BETA
biosensors
biosensor
scanning electron microscopy
PCR
electrophoresis

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