PMID: 8587862Oct 1, 1995Paper

Detection of fetal cells in transcervical samples and prenatal diagnosis of chromosomal abnormalities

Prenatal Diagnosis
M AdinolfiC Rodeck

Abstract

Transcervical samples collected by lavage, aspiration, and cytobrush from women between 6 and 13 weeks of gestation were tested for the presence of fetal cells using fluorescence in situ hybridization (FISH) with probes for chromosomes X, Y, 1, and 21, and by polymerase chain reaction (PCR) amplification of DNA sequences derived from chromosomes X, Y, and 21. With a few exceptions, a good correlation was observed between the results of sexing the fetuses using FISH or PCR on transcervical cell (TCC) samples retrieved by lavage and those obtained by testing fetal (placental) tissue. In a comparative study between TCC samples collected by lavage or cytobrush, the sex of the fetus was correctly diagnosed by PCR amplification of a Y-derived DNA sequence. Variable results were observed with samples obtained by aspiration, mainly because this procedure was found to be more prone to failure to remove thick mucus without previous injection of physiological saline. Chromosome 21-derived small tandem repeats (STRs) of fetal origin were successfully detected in about 40 per cent of TCC samples recovered by lavage. Two cases of chromosomal abnormalities, one of trisomy 21 and one of triploidy, were detected in TCC samples in the course of ...Continue Reading

References

Feb 1, 1991·Genomics·Y NakahoriY Nakagome
Aug 1, 1995·Obstetrics and Gynecology·J KingdomM Adinolfi
Sep 7, 1994·Annals of the New York Academy of Sciences·B PertlM Adinolfi
Mar 1, 1994·Prenatal Diagnosis·M AdinolfiC Rodeck

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Citations

Nov 28, 2002·Prenatal Diagnosis·Cecilia BussaniGianfranco Scarselli
Jun 25, 1998·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·T M KoY P Cheung
Mar 27, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·T ErgInS Batioglu
Jun 29, 2001·Trends in Molecular Medicine·D Wells, J D Delhanty
Feb 1, 1997·Obstetrical & Gynecological Survey·D M ShererM Y Divon
Nov 17, 2006·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Trilochan SahooChristine M Eng
Apr 19, 2008·Obstetrical & Gynecological Survey·F M NdumbeJ C Konje
Apr 1, 1997·Journal of Medical Genetics·J SherlockM Adinolfi
Apr 22, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·Mandy G Katz-JaffeDavid S Cram
Aug 11, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Tal Biron-ShentalAliza Amiel
Jun 1, 1997·Prenatal Diagnosis·M AdinolfiC Rodeck
Feb 8, 2003·Prenatal Diagnosis·Riccardo CioniGianfranco Scarselli
Feb 26, 2005·American Journal of Medical Genetics. Part a·Elissa C OsborneD S Cram
Mar 26, 2005·Prenatal Diagnosis·Riccardo CioniGianfranco Scarselli
Apr 15, 2010·The Analyst·Kang-Yi Lien, Gwo-Bin Lee
Sep 30, 2005·Prenatal Diagnosis·Xiao Xi ZhaoYasuhiko Ozaki
Sep 1, 1996·Prenatal Diagnosis·F MaggiG Simoni
Jan 13, 2006·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Debbie MantzarisGab Kovacs
Apr 8, 2006·Current Opinion in Obstetrics & Gynecology·Farideh Z Bischoff, Joe Leigh Simpson
May 18, 2011·Molecular Biology Reports·Shalu JainSarita Agarwal
Jan 1, 1997·Prenatal Diagnosis·B L Hsi, M Adinolfi

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