Detection of fetal HPCs in maternal circulation after delivery

Transfusion
H OsadaS Sekiya

Abstract

Circulation of mature fetal blood cells in the maternal blood for a certain postpartum period has been verified, but detailed study of the fetal HPCs has not been reported. The objective of this study was to evaluate the frequency and clearance of these cells in the peripheral blood of puerperal women. PBMNCs from 15 puerperal women who gave birth to male infants were cultured in semi-solid medium containing hematopoietic stimulating factors. Colonies formed in the medium were individually characterized, collected, and subjected to PCR amplification of the SRY gene on Y chromosome to confirm fetal origin. The mean numbers of fetal progenitor cell colonies isolated per mL of maternal blood were 1.63, 2.48, 0.56, 0.12, and 0 on the day of delivery, at 4 days, 1 month, 6 months, and 1 year after delivery, respectively. There was no difference in the ratio of fetal versus maternal colonies between erythroid and granulocyte/macrophage lineages. The present study demonstrated that a significant number of fetal HPCs circulate in the maternal blood for a duration of at least 6 months after delivery.

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Citations

Aug 27, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Michele LeducKiarash Khosrotehrani
Feb 12, 2009·Reproductive Sciences·Hongling Du, Hugh S Taylor
May 30, 2009·The Tohoku Journal of Experimental Medicine·Keiichi MatsubaraMasaharu Ito
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sau Nguyen HuuKiarash Khosrotehrani
Feb 26, 2013·Journal of Reproductive Immunology·E SeppanenK Khosrotehrani
Oct 16, 2009·Trends in Molecular Medicine·Thomas Klonisch, Régen Drouin
Nov 28, 2008·La Presse médicale·Olivier ParantSélim Aractingi
Nov 13, 2003·Arthritis and Rheumatism·Kiarash KhosrotehraniDiana W Bianchi
Apr 24, 2008·Journal of the European Academy of Dermatology and Venereology : JEADV·C DroitcourtS Aractingi
Jun 3, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Olivier ParantKiarash Khosrotehrani
Oct 14, 2017·Briefings in Functional Genomics·Anders StåhlbergThomas Kroneis
Nov 16, 2011·Circulation Research·Rina J KaraHina W Chaudhry
Jan 24, 2007·Stem Cell Reviews·Sau Nguyen HuuKiarash Khosrotehrani
Mar 14, 2003·Current Opinion in Obstetrics & Gynecology·Kiarash Khosrotehrani, Diana W Bianchi
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kiarash KhosrotehraniSélim Aractingi
Apr 7, 2005·Journal of Cell Science·Kiarash Khosrotehrani, Diana W Bianchi

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