Detection of FITC-cortisol via modulated supraparticle lighthouses

Analytical Chemistry
Matthew M PetkusM A Hayes

Abstract

Hormones are important bioactive compounds in blood and tissue that vary in concentration in response to stress and certain disease states. Establishing the changes in physiological hormone concentrations over time can lead to more effective diagnoses and perhaps a better understanding of the evolution of stress and disease. To monitor concentration over time, the sampling must be rapid and noninvasive; specimens such as saliva that require little effort to collect are preferred. However, more sensitive assay techniques are needed when compared to blood analysis since free hormone concentration in saliva is only a small fraction of the concentration in circulating blood. In this work, magnetic field-induced structures of paramagnetic particles are used as a solid substrate to demonstrate improved detection limits for a separation-free assay of cortisol. Once formed, the structures are subjected to a rotating magnetic field and this leads to two important features. First is the ability to utilize frequency and phase filtering (lock-in amplification) for the signal generated from surface-bound labeled species. Second is the improved mass transport of the antigen to the surface of the rotating structures. These two unique capabili...Continue Reading

References

Apr 25, 1991·Journal of Immunological Methods·H P Knopf, R Papoian
May 1, 1991·Journal of Clinical Pharmacology·H DerendorfM Krieg
Apr 1, 1994·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·Y H Hwang, X l Wu
Mar 23, 2002·Science·Patrick S DoyleJean-Louis Viovy
Apr 17, 2004·Journal of Immunological Methods·T Krogh-MeibomK L Ingvartsen
May 7, 2004·Biosensors & Bioelectronics·Andrea Prieto AstalanAnatol Krozer
Aug 11, 2005·Physical Review Letters·Nicolas MincKevin D Dorfman

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Citations

May 4, 2013·Biosensors & Bioelectronics·Ariel HechtRaoul Kopelman
Nov 12, 2013·Biosensors & Bioelectronics·Ajeet KaushikShekhar Bhansali
Oct 5, 2013·Biomedical Microdevices·Raiah GottheilMartin Stelzle
Jun 9, 2016·Sensors·Stefan SchrittwieserJoerg Schotter
Nov 4, 2015·Analytical Methods : Advancing Methods and Applications·Christine F Woolley, Mark A Hayes
Feb 18, 2021·Talanta·Sofia M SafarianVladimir P Drachev
Jul 1, 2006·Analytical Chemistry·Amber D Wellman, Michael J Sepaniak

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