Detection of foot-and-mouth disease virus in milk samples by real-time reverse transcription polymerase chain reaction: Optimisation and evaluation of a high-throughput screening method with potential for disease surveillance

Veterinary Microbiology
Bryony ArmsonDonald P King

Abstract

This study aimed to evaluate the utility of milk as a non-invasive sample type for the surveillance of foot-and-mouth disease (FMD), a highly contagious viral disease of cloven-hooved animals. Four milking Jersey cows were infected via direct-contact with two non-milking Jersey cows that had been previously inoculated with FMD virus (FMDV: isolate O/UKG/34/2001). Milk and blood were collected throughout the course of infection to compare two high-throughput real-time reverse transcription polymerase chain reaction (rRT-PCR) protocols with different RT-PCR chemistries. Using both methods, FMDV was detected in milk by rRT-PCR one to two days before the presentation of characteristic foot lesions, similar to detection by virus isolation. Furthermore, rRT-PCR detection from milk was extended, up to 28 days post contact (dpc), compared to detection by virus isolation (up to 14 dpc). Additionally, the detection of FMDV in milk by rRT-PCR was possible for 18 days longer than detection by the same method in serum samples. FMDV was also detected with both rRT-PCR methods in milk samples collected during the UK 2007 outbreak. Dilution studies were undertaken using milk from the field and experimentally-infected animals, where for one sam...Continue Reading

References

Jan 1, 1997·Journal of Virological Methods·R M Armstrong
Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R W RenshawE J Dubovi
Jun 8, 2002·Journal of the American Veterinary Medical Association·Johnny D CallahanWilliam M Nelson
Dec 31, 2002·Journal of Virological Methods·Scott M ReidSoren Alexandersen
Jan 14, 2003·Revue Scientifique Et Technique·R P Kitching
Jul 16, 2003·Journal of Comparative Pathology·S AlexandersenA J M Garland
Apr 16, 2004·Clinical Microbiology Reviews·Marvin J Grubman, Barry Baxt
May 3, 2005·Revue Scientifique Et Technique·A E ShawN P Ferris
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Donald P KingTammy R Beckham
Apr 3, 2007·Journal of Virological Methods·Andrew E ShawDonald P King
Apr 19, 2008·PLoS Pathogens·Eleanor M CottamNick J Knowles
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David J PatonBryan Charleston
Aug 21, 2013·Preventive Veterinary Medicine·T J D Knight-Jones, J Rushton
Apr 29, 2015·Preventive Veterinary Medicine·Nicholas A LyonsPaul E M Fine
Jul 19, 2017·Preventive Veterinary Medicine·Maryam Ansari-LariNezamaddin Azizi

❮ Previous
Next ❯

Citations

Feb 12, 2019·Transboundary and Emerging Diseases·Bryony ArmsonTiziana Lembo
Jan 22, 2020·Transboundary and Emerging Diseases·Bryony ArmsonNicholas A Lyons
Sep 26, 2020·Frontiers in Veterinary Science·Chuan Loo WongWen Siang Tan
Jun 17, 2020·Frontiers in Veterinary Science·Bryony ArmsonNicholas A Lyons
Jun 18, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Marcel SuchowskiReiner Ulrich
Jun 18, 2021·EFSA Journal·UNKNOWN EFSA Panel on Animal Health and Welfare (AHAW)Helen Clare Roberts
Jun 26, 2021·Transboundary and Emerging Diseases·Richard BradhurstKeith Sumption

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleic acid extraction
Assay
PCR

Software Mentioned

Prism
R
GraphPad
fmsb

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.