Detection of free and plankton-associated Helicobacter pylori in seawater

Journal of Applied Microbiology
L CelliniG Donelli

Abstract

To detect both free and plankton-associated Helicobacter pylori in seawater samples collected on the Italian coast of the Adriatic Sea using a nested-PCR. Dissolved oxygen, pH, salinity and chlorophyll 'a' were the parameters recorded together with the characterization of zooplanktonic organisms. Plankton-associated H. pylori DNA was searched for in water samples filtered through 200 and 64 microm nylon nets whereas free bacteria were retained with the subsequent filtration through 0.22 microm pore-size membranes. Nested-PCR using primers for the glmM (ureC) gene was performed to reveal the presence of H. pylori. The DNA sequencing of amplified products confirmed the specificity of the assay. The sensitivity of the nested-PCR assay for H. pylori detection was 62 CFU per 100 ml in spiked water samples. Helicobacter pylori either free or bound to planktonic organisms was found in seven of 12 monthly samples. In particular, free bacteria were detected during the summer sampling and in November, December and March associated to planktonic cells. The presence of free and plankton-associated H. pylori in seawater suggests that it can be a significant reservoir and a potential route of transmission for the microorganism. Our study see...Continue Reading

References

Aug 1, 1991·Journal of Bacteriology·L NilssonS Kjelleberg
Jan 1, 1994·Microbiology and Immunology·L CelliniB Dainelli
May 1, 1994·British Heart Journal·M A MendallT C Northfield
Jan 1, 1994·Microbiology and Immunology·L CelliniB Dainelli
Apr 1, 1996·Gastroenterology·K HultenF A El-Zaatari
Jun 1, 1996·Comparative Immunology, Microbiology and Infectious Diseases·S DumontetS Vanucci
Jun 1, 1997·Journal of Bacteriology·H De ReuseD Mengin-Lecreulx
Jul 15, 1998·Current Microbiology·C W BrasherA K Bej
Jul 23, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L CelliniB Dainelli
Nov 13, 1998·FEMS Microbiology Letters·G DonelliL Cellini
Mar 2, 1999·Applied and Environmental Microbiology·R Tarsi, C Pruzzo
Sep 29, 1999·Advances in Microbial Physiology·M R Barer, C R Harwood
Dec 14, 1999·Journal of Applied Microbiology·J P HegartyK H Baker
Dec 22, 1999·JAMA : the Journal of the American Medical Association·J ParsonnetT Haggerty
Mar 29, 2001·Digestive Diseases and Sciences·L CelliniL Artese
Sep 1, 2001·Microbiology and Immunology·T HoriuchiY Eishi
Oct 2, 2001·Archives of Medical Research·M Mazari-HiriartA Cravioto
Dec 1, 2001·Scandinavian Journal of Infectious Diseases·K H Baker, J P Hegarty
Jan 5, 2002·International Journal of Hygiene and Environmental Health·J MooreB Millar
Jan 5, 2002·Applied and Environmental Microbiology·Hans-Olof NilssonTorkel Wadström
Jan 26, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·M EguchiN Miyamoto
Feb 19, 2002·Alimentary Pharmacology & Therapeutics·M F Go
Feb 20, 2002·Journal of Applied Microbiology·M M LleòP Canepari
Mar 2, 2002·Applied and Environmental Microbiology·Yingzhi LuKaren Goodman

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Citations

Dec 25, 2007·Applied and Environmental Microbiology·John C WilliamsTraci L Testerman
Feb 5, 2008·Applied and Environmental Microbiology·N F AzevedoM J Vieira
Jul 22, 2008·Canadian Journal of Microbiology·Françoise BichaiBenoit Barbeau
Apr 23, 2013·TheScientificWorldJournal·Ahmad Reza BahramiHajieh Ghasemian Safaei
Jan 31, 2016·Antonie van Leeuwenhoek·Milagro Fernández-DelgadoPaula Suárez
Apr 30, 2010·International Journal of Hygiene and Environmental Health·S LinkeJ Gebel
Feb 4, 2010·International Journal of Food Microbiology·F F Vale, J M B Vítor
Apr 16, 2008·Journal of Applied Microbiology·L CelliniO Trubiani
Jun 16, 2015·Frontiers in Public Health·Mahnaz Mazaheri AssadiAnwar Huq
Mar 15, 2015·Archives of Oral Biology·Negin AmiriAmirhooshang Alvandi
May 21, 2009·FEMS Immunology and Medical Microbiology·Leif Percival Andersen, Lone Rasmussen
Nov 21, 2018·Foodborne Pathogens and Disease·Maria Consuelo Pina-PérezMaria Antonia Ferrús
Apr 3, 2007·Applied and Environmental Microbiology·N F AzevedoM J Vieira
Aug 23, 2018·World Journal of Gastroenterology : WJG·Nicoletta C Quaglia, Angela Dambrosio
May 11, 2018·Microbiology and Molecular Biology Reviews : MMBR·Skander HathroubiKaren M Ottemann
Nov 2, 2019·International Journal of Food Microbiology·Nicoletta C QuagliaAngela Dambrosio

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