PMID: 12761921May 24, 2003Paper

Detection of GABAA alpha 2 mRNA in rat vestibular end organ with in-situ hybridization

Zhonghua er bi yan hou ke za zhi
H ZhengY Fu

Abstract

To investigate the effect of the gamma-aminobutyric acid (GABA) on rat vestibular end organ. In the rat inner ear paraffin slides, GABAA receptor alpha 2 subunit mRNA in vestibular end organ has been detected with in-situ hybridization. Digoxigenin-GABAA alpha 2 cDNA prob (549 base pair), Anti-Digoxigenin-AP (Fab fragments) and BM Purple AP Substrate (precipitating) have been used. GABAA receptor alpha 2 subunit mRNA has been found in all vestibular ganglion cells and nerve ending surrounding type I hair cells in rat's cristae ampullaris. GABAA alpha 2 has not been found in hair cells and supporting cells. As a positive control, GABAA alpha 2 mRNA has been found in Purkinje cells and granule cells in rat's cerebellum. GABAA alpha 2 has not been found in OMP negative control, non-prob negative control and non-anti-digoxingenin control in inner ear and cerebellum slides. GABAA alpha 2 receptor has been found in vestibular afferent nerve endings and vestibular ganglion cells. It strongly indicates that GABA is an afferent nerve transmitter in the vestibular end organ and plays an important role in the vestibular afferent nerve transmission.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved