Detection of genes encoding virulence factors and bacteriocins in fecal enterococci of poultry in Portugal

Avian Diseases
Patricia PoetaC Torres

Abstract

Seventy-six Enterococcus isolates (43 E. faecalis, 30 E. faecium, two E. durans, and one E. hirae) recovered from fecal samples of poultry in a slaughterhouse (one isolate per fecal sample and one fecal sample per lot of animals) were studied for bacteriocin production and for the presence of genes encoding bacteriocins and virulence factors. The presence of genes encoding virulence factors (cpd, geE, fsr, ace, agg, and esp) and bacteriocins (entA, entB, entP, entQ, entAS-48, entL50A/B, cyl, and bac31) were studied by polymerase chain reaction in all enterococci. At least two virulence genes were detected in all 43 E. faecalis isolates, cpd and gelE being the most frequently detected genes (97.7%) followed by ace (62.8%), agg (39.5%), fsr (27.9%), and esp (2.3%). No virulence genes were detected in the other enterococcal species with the exception of one E. faecium and one E. durans isolates that harbored the gelE gene. Antimicrobial activity against eight indicator bacteria (including Listeria monocytogenes) was assayed in the enterococci, and 23 (30.3%) showed inhibitory activity against L. monocytogenes, the other 22 enterococci showing activity against indicator bacteria other than L. monocytogenes. Only the entA, entB, and...Continue Reading

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Citations

Jun 5, 2016·Comparative Immunology, Microbiology and Infectious Diseases·Manuela OliveiraTeresa Semedo-Lemsaddek
May 31, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·P PoetaC Torres

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Methods Mentioned

BETA
electrophoresis
PCR
PCRs

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