Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping).

Journal of Clinical Microbiology
O OkwumabuaM M Chengappa

Abstract

Whole-cell chromosomal digests of 54 isolates of Streptococcus suis encompassing all known serotypes from a geographically varied collection were examined by PstI restriction fragment length polymorphisms and then hybridized with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from a mixture of 16S and 23S rRNAs from Escherichia coli MRE600. The hybridization patterns showed genetic heterogeneity within and between S. suis serotypes. Most isolates (87%) representing 28 serotypes contained a common band at approximately 1.8 kb. However, 13% of the isolates representing seven serotypes lacked the 1.8-kb band, indicating that the species as currently defined is diverse. Nonetheless, the 1.8-kb band may be a useful genotypic marker for identification of most S. suis isolates. We tested the ability of this technique to discriminate between virulent and avirulent S. suis type 2 isolates. A virulent strain of S. suis type 2 could be distinguished from avirulent strains by the presence of specific bands. No correlation was obvious between band pattern and hemolysin production.

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Citations

Apr 9, 2005·Clinical and Diagnostic Laboratory Immunology·Ogi Okwumabua, Sharmila Chinnapapakkagari
Feb 9, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ogi OkwumabuaMelissa Behr
May 10, 2020·Microbiology Resource Announcements·Ogi OkwumabuaJason W Sahl
Jul 28, 2001·Journal of Applied Microbiology·K B Joung, J C Côté
Feb 6, 2020·Pathogens·Rujirat HatrongjitAnusak Kerdsin
Mar 10, 2001·Clinical and Diagnostic Laboratory Immunology·O OkwumabuaP G Reddy
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Mar 7, 2003·FEMS Microbiology Letters·Makoto OsakiTsutomu Sekizaki
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