Detection of glia-derived nexin in the olfactory system of the rat.

Neuron
E. ReinhardD Monard

Abstract

Glia-derived nexin (GDN) is a 43 kd cell-secreted protease inhibitor with neurite promoting activity. We have raised specific polyclonal antisera to rat GDN. These antibodies stain a single band at 43 kd on immunoblots of concentrated C6 glioma-conditioned medium and have been used to demonstrate that GDN is present in the olfactory system of the rat. One band at 43 kd is recognized by the GDN antibodies on immunoblots of olfactory bulb homogenate. Immunohistochemistry shows that GDN occurs predominantly in the olfactory nerve layer of the olfactory bulb and in the olfactory submucosa. Comparative studies with antibodies against vimentin, GFAP, and fibronectin suggest that anti-GDN recognizes cells associated with the olfactory system, but not exclusively the olfactory neurons themselves. Data from the immunohistochemical studies were confirmed by RNA blots and GDN mRNA expression throughout development of the olfactory bulb. The high levels of GDN in the rat olfactory system may be related to the continuous degeneration and regeneration phenomena taking place in these structures.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1987·Gene Analysis Techniques·C B Harley
Jan 1, 1988·Developmental Neuroscience·A D ZurnD Monard
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B L BergmanJ B Baker
Jan 1, 1987·Archives of Biochemistry and Biophysics·S R StoneD Monard
Jan 1, 1986·Journal of Biochemical and Biophysical Methods·S A KrawetzG H Dixon
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D MonardR Gysin
Jan 1, 1973·Tissue & Cell·P P Graziadei
Jun 4, 1974·Biochemistry·V GlisinC Byus
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1983·Progress in Brain Research·D MonardF Solomon

❮ Previous
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Citations

May 6, 1999·Journal of Cellular Physiology·C MbebiM Verdière-Sahuqué
Jan 1, 1992·Archives of Insect Biochemistry and Physiology·E H Hanneman, M R Kanost
Mar 29, 2002·The Journal of Comparative Neurology·Winnie W AuCharles A Greer
Jan 1, 1990·Journal of Cellular Physiology·D Gurwitz, D D Cunningham
Oct 15, 1992·Microscopy Research and Technique·F Miragall, R Dermietzel
Jan 1, 1994·Journal of Neuroscience Research·K L BeecherB W Festoff
Feb 1, 1994·Journal of Neuroscience Research·E ReinhardD Monard
Dec 18, 2001·Journal of Neurobiology·M Z LevineC D Derby
Dec 1, 1990·Cell and Tissue Research·W HalfterD Monard
Dec 24, 2005·Journal of Neurocytology·Adele J VincentMeng Inn Chuah
Sep 17, 1993·Brain Research. Developmental Brain Research·S L BirocJ M Fisher
Dec 1, 1995·Neuroscience Research·M NishiboriK Saeki
Apr 11, 1994·Neuroscience Letters·C S SimpsonB J Morris
Dec 28, 1989·Gene·J SommerD Monard
Jul 17, 1998·Brain Research Bulletin·A Ramón-Cueto, J Avila
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L WagnerD D Cunningham
Aug 20, 2003·Human Molecular Genetics·Florence MolinariLaurence Colleaux
May 1, 1990·Journal of Neurochemistry·K P CavanaughR A Bradshaw
Jan 1, 1991·Journal of Neurochemistry·S L WagnerD D Cunningham
Dec 31, 1992·Annals of the New York Academy of Sciences·D D Cunningham
Dec 31, 1992·Annals of the New York Academy of Sciences·D MonardC Nitsch
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·R Bar-ShavitI Vlodavsky
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·R G Sitrin
Aug 3, 2011·Reproductive Biology and Endocrinology : RB&E·Schu-Rern ChernEdmund I Tsuen Chen
Nov 15, 1992·The Journal of Comparative Neurology·F MiragallR Dermietzel
May 2, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lei ChenRunlin Z Ma
Jul 29, 2004·Journal of Cellular Physiology·Benjamin RichardMarie-Christine Bouton
Mar 6, 2003·The Anatomical Record. Part B, the New Anatomist·J G BoydR Doucette
May 15, 1991·Biochemical and Biophysical Research Communications·R W MeierA Tobler

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