Detection of glutathione in whole blood after stabilization with N-ethylmaleimide

Analytical Biochemistry
Daniela GiustariniRanieri Rossi

Abstract

The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20°C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.

References

Mar 29, 2001·Free Radical Biology & Medicine·I NémethD Boda
Jun 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anna PastoreFiorella Piemonte
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Dec 4, 2003·Free Radical Biology & Medicine·Daniela GiustariniRanieri Rossi
Mar 31, 2004·Molecular Aspects of Medicine·Virginia B C JunqueiraGuilherme P Deucher
May 13, 2006·Clinical Chemistry·Ranieri RossiDaniela Giustarini
Jan 27, 2009·Biological Chemistry·Nazzareno BallatoriChristine L Hammond
May 16, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ranieri RossiIsabella Dalle-Donne
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa
Dec 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Daniela GiustariniRanieri Rossi

❮ Previous
Next ❯

Citations

May 11, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tereza MooreTina M Cowan
Aug 10, 2013·Nature Protocols·Daniela GiustariniRanieri Rossi
Sep 6, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rana RaisBarbara S Slusher
Feb 26, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniela GiustariniRanieri Rossi
Feb 21, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniela GiustariniRanieri Rossi
Jan 22, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Khaled KhazimPaolo Fanti
Oct 18, 2015·Free Radical Biology & Medicine·Daniela GiustariniRanieri Rossi
Oct 13, 2012·Biosensors & Bioelectronics·Hailong LiErkang Wang
Oct 4, 2014·Scandinavian Journal of Gastroenterology·Åse Florholmen-KjærKim Erlend Mortensen
Nov 7, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yinhuan LiRuyi Zhang
May 4, 2017·Journal of Clinical Medicine·Gregory M Enns, Tina M Cowan
Oct 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zaneta BuchtovaOndrej Zitka
Sep 19, 2012·The Journal of Pharmacology and Experimental Therapeutics·Krystyna M WozniakBarbara S Slusher
Jan 16, 2019·Scientific Reports·Xiaoyun FuJosé A López
Jul 9, 2020·Apoptosis : an International Journal on Programmed Cell Death·Mehrdad GhashghaeiniaMartin Köberle
May 14, 2021·Chembiochem : a European Journal of Chemical Biology·Yibin ZhangHaiying Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.