Detection of gonadotropin-releasing hormone receptor and its mRNA in primary human epithelial ovarian cancers

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
C-H ChienS-N Chow

Abstract

The hypothalamic neuropeptide gonadotropin-releasing hormone (GnRH) serves a key role in regulating mammalian reproductive function. An extrapituitary role for GnRH in the normal and malignant reproductive tissues has been postulated. The purpose of our study is to demonstrate the presence and levels of GnRH receptor (RGnRH) protein and its mRNA in normal and malignant tissues of ovary. Normal human ovarian tissues (n = 13), as well as epithelial ovarian cancer specimens from stages I-IV (n = 39), were obtained from appropriate patients at operation room. Monoclonal antibodies against RGnRH were used for immunohistochemical evaluation of paraffin-embedded ovarian tissue sections by methods of streptavidin-biotin immunostaining. The molecular size and levels of RGnRH were determined by enhanced chemiluminescence-Western blot assay. The amount of RGnRH mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The rate of positive immunostaining in ovarian cancers was 53.8% (21/39). The rate of positive staining in the late stage (stages III and IV) was significantly higher than that in the early stage (stages I and II). A single band of molecular weight of about 60 kDa was detected from protein extracts of ov...Continue Reading

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Citations

Oct 18, 2006·Bioorganicheskaia khimiia·S V BurovM Fridkin
Apr 29, 2014·Taiwanese Journal of Obstetrics & Gynecology·Kuan-Hao TsuiChih-Ping Chen
Oct 31, 2008·The FEBS Journal·Lydia W T Cheung, Alice S T Wong
Dec 10, 2019·Journal of Labelled Compounds & Radiopharmaceuticals·Richard FjellakselRune Sundset

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