Detection of grlA and gyrA mutations in 344 Staphylococcus aureus strains.

Antimicrobial Agents and Chemotherapy
T WangK Sato

Abstract

Mutations in the grlA and gyrA genes of 344 clinical strains of Staphylococcus aureus isolated in 1994 in Japan were identified by combinations of single-strand conformation polymorphism analysis, restriction fragment length analysis, and direct sequencing to identify possible relationships to fluoroquinolone resistance. Five types of single-point mutations and four types of double mutations were observed in the grlA genes of 204 strains (59.3%). Four types of single-point mutations and four types of double mutations were found in the gyrA genes of 188 strains (54.7%). Among them, the grlA mutation of TCC-->TTC or TAC (Ser-80-->Phe or Tyr) and the gyrA mutation of TCA-->TTA (Ser-84-->Leu) were principal, being detected in 137 (39.8%) and 121 (35.9%) isolates, respectively. The grlA point mutations of CAT-->CAC (His-77 [silent]), TCA-->CCA (Ser-81-->Pro), and ATA-->ATT (Ile-100 [silent]) were novel, as was the GAC-->GGC (Asp-73-->Gly) change in gyrA. A total of 15 types of mutation combinations within both genes were related to ciprofloxacin resistance (MIC > or = 3.13 microg/ml) and were present in 193 mutants (56.1%). Strains containing mutations in both genes were highly resistant to ciprofloxacin (MIC at which 50% of the iso...Continue Reading

References

Sep 1, 1994·Antimicrobial Agents and Chemotherapy·H ItoS Nakamura
May 1, 1993·Antimicrobial Agents and Chemotherapy·G W KaatzC A Ruble
Dec 1, 1996·Antimicrobial Agents and Chemotherapy·F BlancheJ Crouzet

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Citations

Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Apr 24, 2003·Antimicrobial Agents and Chemotherapy·F MasselotD Raoult
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·M TanakaK Sato
Jan 26, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·M TanakaK Sato
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·David C. Hooper
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Jeffrey J CampionMartin E Evans
Jun 9, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Siddhartha RoychoudhuryMing Chu Hsu
Aug 30, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Marzieh AligholiMohammad Emaneini
May 27, 2005·Antimicrobial Agents and Chemotherapy·Jeffrey J CampionMartin E Evans

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