Detection of haemoglobin using an adsorption approach at a liquid-liquid microinterface array

Analytical and Bioanalytical Chemistry
Eva Alvarez de EulateDamien W M Arrigan

Abstract

The behaviour of haemoglobin (Hb) at the interface between two immiscible electrolyte solutions (ITIES) has been examined for analytical purposes. When Hb is fully protonated under acidic conditions (pH <pI) in the aqueous phase, it undergoes a potential-dependent adsorption and complexation, at the interface, with the anions of the organic phase electrolyte. When utilised as a simple and fast preconcentration step, consisting of adsorbing the protein at the interface, in conjunction with voltammetric desorption, this opens up the ITIES to the adsorptive stripping voltammetry approach. Utilising a 60 s adsorption step and linear sweep voltammetry, a linear response to Hb concentration in aqueous solution over the range 0.01-0.5 μM was achieved. The equation of the best-fit straight line was I(p) = 7.46 C - 0.109, R = 0.996, where I(p) is the peak current (in nanoampere) and C is haemoglobin concentration (in micromolar). The calculated detection limit (3σ) was 48 nM for a 60 s preconcentration period, while the relative standard deviation was 13.3% for six successive measurements at 0.1 μM Hb. These results illustrate the prospects for simple, portable and rapid label-free detection of biomacromolecules offered by electrochemis...Continue Reading

References

Mar 17, 2007·Journal of Medical Engineering & Technology·J ShiY Fang
Dec 25, 2007·Chemical Reviews·Joseph Wang
Jun 26, 2008·Analytical Chemistry·Micheál D ScanlonDamien W M Arrigan
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Francine KivlehanDamien W M Arrigan
Jan 9, 2009·Analytical and Bioanalytical Chemistry·Axel Warsinke
Mar 24, 2009·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Dec 17, 2009·Analytical Chemistry·Grégoire HerzogDamien W M Arrigan
Dec 17, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Goce DimeskiAndrew St John
May 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Toshiyuki OsakaiHajime Katano
Jun 11, 2010·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Aug 6, 2010·Analytical and Bioanalytical Chemistry·Shigeru AmemiyaBenjamin Kabagambe
Oct 1, 2010·Physical Chemistry Chemical Physics : PCCP·José A RibeiroCarlos M Pereira

Related Concepts

Adsorption
Bos indicus
Electrolytes
Eryhem
Electrochemical Techniques
Limit of Detection
Hemoglobin
Solution Hybridization
Detection
Liquid Dosage Form

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