Detection of HBsAg mutants

Journal of Medical Virology
Carla Osiowy

Abstract

HBsAg screening is carried out routinely to detect hepatitis B virus (HBV) infection. The immunoassays used employ capture antibodies often having specificity for epitopes present on the antigenic (a) determinant of the HBsAg. Loss of detection may occur due to mutations within and/or outside of the a determinant that affect conformational epitope recognition or HBsAg secretion or expression. Most of the mutations associated with immune escape occur within the second loop of the a determinant. In order to detect these HBsAg mutants, antibodies to subdominant regions within the a determinant or outside of the HBsAg may be required, and this has been the focus of many recent studies. Any changes to immunoassay formulations should also address the possible effect of HBV genotypic polymorphisms on assay specificity and sensitivity. HBsAg mutants may also be identified through nucleic acid detection of HBV in serum. Various molecular analysis methods have been developed to provide specific and sensitive detection of HBsAg mutants, including sequencing, limiting dilution cloning PCR (LDC-PCR), gap ligase chain reaction (gLCR), and real time PCR. Sequencing the HBsAg coding region provides specific information on the nucleotide sequen...Continue Reading

References

Jun 29, 2002·Journal of Clinical Microbiology·Carla Osiowy
Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Manna ZhangGerald Y Minuk
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tatyana KalininaMartina Sterneck
Dec 2, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Bernard WeberWalter Melchior
Dec 21, 2004·World Journal of Gastroenterology : WJG·Zhong-Liang HuChun-Yan Fu
Jan 18, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Bernard Weber
Mar 22, 2005·Journal of Medical Virology·Varsha ThakurShiv K Sarin
Apr 6, 2005·World Journal of Gastroenterology : WJG·Wei-Guo HuZu-Chuan Zhang

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Citations

Nov 30, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Robério A A Pondé
Sep 10, 2010·Journal of Clinical Microbiology·Marie GauthierGuy Vernet
Apr 25, 2012·Journal of Global Infectious Diseases·Disha A PatelMahendra M Vegad
Jun 3, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Carla S CoffinM John Gill
Jul 20, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Szu-Ming ChenHai-Lung Wang
Dec 27, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Anna Jeffery-SmithCheuk Yan William Tong
Mar 26, 2009·Journal of Medical Virology·Antigoni KatsoulidouAngelos Hatzakis
Apr 20, 2006·Journal of Medical Virology·Gerald Schochetman, Mary C Kuhns
Jan 21, 2014·Journal of Viral Hepatitis·X Huang, F B Hollinger
Jun 25, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R A A Pondé
Sep 24, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ling-Ling LuChunfeng Qu
Aug 24, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zunaira AwanBisma Rauff
Jul 29, 2016·Journal of Medical Virology·Massimo De PaschalePierangelo Clerici
Aug 23, 2018·International Journal of Molecular Medicine·Tong WangJun Cheng

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