Detection of HBV genome in the plasma and peripheral blood mononuclear cells of Iranian HBsAg negative patients with HIV infection: occult HBV infection

Archives of Virology
Zahra TajikAtousa Fakhim

Abstract

The presence of hepatitis B virus (HBV) DNA in the absence of traceable hepatitis B surface antigen (HBsAg) in the plasma specimen of patients is defined as occult HBV infection (OBI). This study aimed to detect HBV-DNA in the plasma and peripheral blood mononuclear cells (PBMCs) of Iranian HBsAg negative patients with human immunodeficiency virus (HIV) infection. This cross-sectional study was conducted on 172 patients with HIV infection from September 2015 to August 2017. The patients were tested for serological parameters (HBsAg, HBcAb, HBeAg and HBeAb) against HBV infection. Moreover, they were tested for HBV viral load (using COBAS TaqMan 48 Kit, Roche, USA) in plasma and the presence of the HBV genome in PBMC specimens using real-time PCR. The mean age of the patients was 35.4 ± 13.4 years. Of the 172 studied patients, 109 (63.4%) were male. In this study, 151 (87.8%) patients were negative for HBsAg, 111 (64.5%) patients were negative for all HBV infection serological markers, 9 (5.2%) patients were only positive for HBsAg and 29 (16.9%) patients were only positive for HBcAb. Moreover, five (3.3%) patients with HBsAg negative had OBI (in the plasma sample of four patients and PBMC specimens of all five patients, HBV-DNA ...Continue Reading

References

Apr 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B A KoblinC E Stevens
Feb 1, 1993·The Journal of Infectious Diseases·S J TwuJ Phair
Mar 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J KöckH J Schlicht
Mar 25, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M HoferM Opravil
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J F ColinP Marcellin
Jun 26, 2002·Journal of Viral Hepatitis·Ke-Qin Hu
Dec 31, 2003·Gastroenterology·Teresa PollicinoGiovanni Raimondo
Jun 24, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Norah J ShireKenneth E Sherman
Sep 14, 2004·Revista Da Sociedade Brasileira De Medicina Tropical·Milta Gomes de SouzaLuis Everton Esmeraldino
Dec 14, 2004·Revista Da Sociedade Brasileira De Medicina Tropical·Maria Rita de Cassia Costa MonteiroJosé Fernando de Castro Figueiredo
Feb 8, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·I Chemin, C Trépo
Jan 9, 2007·World Journal of Gastroenterology : WJG·Stephan Schaefer
Aug 21, 2007·Journal of Medical Virology·Gaia NebbiaAnna Maria Geretti
Aug 22, 2008·Journal of Hepatology·Giovanni RaimondoFabien Zoulim
Nov 4, 2010·Journal of Infection in Developing Countries·Amitis RamezaniArezoo Aghakhani
Aug 10, 2012·Journal of Immigrant and Minority Health·Fabio TramutoFrancesco Vitale
May 29, 2014·Journal of Tropical Medicine·Oluyinka Oladele OpaleyeO Adekunle Olowe
Dec 3, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mukhlid YousifAnna Kramvis
Sep 20, 2015·International Journal of STD & AIDS·Arezoo AghakhaniAmitis Ramezani
Jun 4, 2016·Medical Journal, Armed Forces India·A K SoodManish Manrai
Jan 5, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Guo-Feng ChenGeorge Lau

❮ Previous
Next ❯

Citations

Nov 6, 2018·Archives of Virology·Maryam SheikhHossein Keyvani
Sep 10, 2020·Current HIV Research·Mohsen MoghoofeiSeyed Hamidreza Monavari

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.