PMID: 8963384Dec 1, 1995Paper

Detection of Helicobacter pylori DNA in gastric juice by the polymerase chain reaction: comparison with findings in bacterial culture and the detection of tissue IgA and serum IgG antibodies against Helicobacter pylori

Journal of Gastroenterology
N MatsukuraM Ohbayashi

Abstract

The detection of Helicobacter pylori in gastric juice by the polymerase chain reaction (PCR) was undertaken in 124 patients with peptic ulcer or chronic gastritis. PCR products were evaluated by agarose gel electrophoresis and Southern hybridization of H. pylori-specific DNA sequences. Positive and negative results of the PCR analysis in 72 examinations were compared with those from bacterial culture, and with the detection of tissue IgA antibody against H. pylori by enzyme-linked immunosorbent assay ELISA; Serion, Wuerzburg, Germany, and detection of serum IgG antibody against H. pylori by ELISA; Radim Pomezia, Italy. Thirty-four PCR-positive samples evaluated by electrophoresis and hybridization coincided with positive samples in 56% of bacterial cultures, 59% of tissue IgA antibody identifications, and 94% of serum IgG antibody evaluations; 26 PCR-negative samples coincided with negative samples in 96% of bacterial cultures, 81% of tissue IgA antibody evaluations, and 38% of serum IgG assessments. We compared the detection achieved with the H. pylori PCR assay in gastric juice with that in biopsies taken from the antrum and upper corpus in 90 examinations, and found them to be both positive in 34 (38%) and 36 (40%) of specim...Continue Reading

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Citations

Apr 28, 2001·FEMS Microbiology Letters·M G Domínguez-BelloP Gueneau de Novoa
Jun 19, 2001·Diagnostic Microbiology and Infectious Disease·N PachecoM G Domínguez-Bello
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May 10, 2016·Journal of Clinical Gastroenterology·Hólmfridur HelgadóttirEinar S Björnsson
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Sep 28, 2004·Journal of Pediatric Gastroenterology and Nutrition·Tsutomu YamazakiTakaaki Matsumoto
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Apr 18, 2019·Journal of Clinical Gastroenterology·Hólmfridur HelgadóttirEinar S Björnsson
Aug 10, 2004·Alimentary Pharmacology & Therapeutics·N MatsukuraN Yamada
Dec 18, 2020·Scandinavian Journal of Gastroenterology·Hólmfríður HelgadóttirEinar S Björnsson
Sep 9, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Zehua ChenKun Yang

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