Detection of helicobacter pylori in bronchoalveolar lavage of patients with chronic obstructive pulmonary disease by real time polymerase chain reaction

Jundishapur Journal of Microbiology
Mitra Samareh FekriHamid Reza Mollaie

Abstract

Chronic obstructive pulmonary disease (COPD) is one of the most important causes of disability and mortality in the world. Although cigarette smoking and environmental pollutants have been recognized as the major causes of COPD, the role of infection in the pathogenesis and progression of COPD has also been reported. The aim of the present study was to find the relationship between Helicobacter Pylori infection and COPD through anti H. pylori IgG serology, real time PCR of bronchoalveolar lavage and trans bronchial biopsy urease tests. This descriptive cross-sectional study was carried out on 60 adults with COPD. After obtaining the patient's history, physical examination, spirometry and confirmation of COPD diagnosis by pulmonologist, subjects were selected through convenience sampling. In order to determine the severity and prognosis of disease, the global initiative for chronic obstructive lung disease (GOLD) criteria and BODE index were used. Subjects underwent bronchoscopy for obtaining bronchoalveolar lavage (BAL) samples and biopsy was performed. Biopsy and BAL samples were investigated respectively by urease test and real time PCR. Moreover, patients' serum samples were serologically studied for detection of anti H. pyl...Continue Reading

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Citations

Sep 16, 2015·Helicobacter·Francesco FranceschiJannis Kountouras
Mar 23, 2017·World Journal of Gastroenterology : WJG·Magdalena ChmielaPaweł Stączek
Feb 1, 2017·Jundishapur Journal of Microbiology·Mitra Samareh-FekriArshia Jamali Paghalhe
Feb 19, 2020·Molecular Genetics & Genomic Medicine·Abolfazl Zendehdel, Maryam Roham

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Methods Mentioned

BETA
PCR
bronchoalveolar lavage
bronchoalveolar
biopsy
enzyme linked immunosorbent assay
enzyme linked immunosorbent

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