PMID: 8188812Feb 1, 1994Paper

Detection of hepatitis A virus in clinical and environmental samples by immunomagnetic separation and PCR

Journal of Virological Methods
C Monceyron, B Grinde

Abstract

Magnetic beads coated with antibodies against surface epitopes of the hepatitis A virus (HAV) captured efficiently viral particles from various types of samples. Contaminating substances, including particulate material, were removed by using a magnet to retain the beads during the washing procedure. A sensitive reverse transcriptase/nested polymerase chain reaction (RT-PCR) was developed to confirm the presence of viral particles on the beads. The above technique was shown to be useful for the detection of a laboratory strain of HAV added to polluted river water, sea water and fecal extracts.

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Citations

Nov 1, 1996·Indian Journal of Pediatrics·S D ChitambarV A Arankalle
May 15, 2012·Analytical and Bioanalytical Chemistry·Ivo SafarikMirka Safarikova
Aug 29, 2000·Journal of Virological Methods·S BidawidS A Sattar
Feb 19, 2002·Journal of Virological Methods·L Bakkali KassimiC Cruciere
Oct 8, 1998·Journal of Virological Methods·J W LiF H Chao
Jun 17, 1997·International Journal of Food Microbiology·D HäfligerP Hübner
May 26, 2012·TheScientificWorldJournal·Laura Cristina BerumenGuadalupe García-Alcocer
Jan 15, 2003·Clinical Microbiology Reviews·Dale W GriffinJoan B Rose
Jun 1, 2012·International Journal of Peptides·Brian HallKeith K Parker
Sep 17, 2011·International Journal of Hygiene and Environmental Health·Ibrahim Ahmed HamzaMichael Wilhelm
Mar 30, 2011·PloS One·Mary E PiperSonja R Gerrard
Nov 27, 2018·Journal of Food Protection·Sang-Mu KoDuwoon Kim
Feb 17, 1998·Applied and Environmental Microbiology·N JothikumarT W Mariam
Aug 9, 2021·Talanta·Laura MicheliDanila Moscone

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