PMID: 8188812Feb 1, 1994Paper

Detection of hepatitis A virus in clinical and environmental samples by immunomagnetic separation and PCR

Journal of Virological Methods
C Monceyron, B Grinde


Magnetic beads coated with antibodies against surface epitopes of the hepatitis A virus (HAV) captured efficiently viral particles from various types of samples. Contaminating substances, including particulate material, were removed by using a magnet to retain the beads during the washing procedure. A sensitive reverse transcriptase/nested polymerase chain reaction (RT-PCR) was developed to confirm the presence of viral particles on the beads. The above technique was shown to be useful for the detection of a laboratory strain of HAV added to polluted river water, sea water and fecal extracts.


Feb 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·P J Provost, M R Hilleman
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