Detection of hepatitis A virus RNA in oyster meat.

Applied and Environmental Microbiology
T L CromeansH S Margolis

Abstract

Detection of low concentrations of viruses in shellfish is possible with nucleic acid amplification by PCR. Hepatitis A virus (HAV) has been detected in oyster meat by reverse transcription-PCR (RT-PCR). We developed a method to identify HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation, or injection. With dot blot hybridization detection of amplicons from the RT-PCR, rapid screening of a large number of samples is feasible. As few as 8 PFU of HAV/g of oyster meat can be detected.

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Citations

Aug 8, 2020·Journal of Clinical Microbiology·Swinburne A J AugustineTimothy J Wade
Jan 19, 2006·Clinical Microbiology Reviews·Omana V NainanHarold S Margolis
Sep 25, 2003·Journal of Food Protection·A Di PintoC Buonavoglia
Sep 21, 2011·Letters in Applied Microbiology·A NamsaiR Ittapong
Jan 11, 2000·Journal of Food Protection·P R Leggitt, L A Jaykus
Aug 5, 2000·Applied and Environmental Microbiology·F Le GuyaderM Pommepuy
Sep 12, 2019·Microorganisms·Christine L DensmoreLuke R Iwanowicz
Aug 18, 2000·International Journal of Food Microbiology·D Lees

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