PMID: 9441800Feb 7, 1998Paper

Detection of hepatitis B virus x transcripts in human hepatocellular carcinoma tissues

The Journal of Surgical Research
S KobayashiK Isono

Abstract

The hepatitis B virus (HBV) or hepatitis C virus (HCV) sequences were frequently detected in hepatocellular carcinoma (HCC) tissues by the method of polymerase chain reaction (PCR). However, the expression levels of HBV and HCV in HCC tissues remain to be documented. This study evaluates the mRNA expression levels of those virus sequences in the human HCC tissues through Northern blot hybridization. We performed Northern blot hybridization to identify the HBV x mRNA and HCV RNA in HCC tissues of 14 cases excised at Chiba University Hospital. The HBV x transcripts could be identified in the tumor tissues from all three serum HBsAg-positive cases and two cases among 11 HBsAg-negative patients. One HBsAg-negative case showed the most intensive signal in those five cases. However, HCV RNA could not be identified by this method. The HBV x gene transcript is predicted to have a size of about 0.7 kb; however, the molecular weight of HBV x specific mRNA is not consistent and is distributed between approximately 2.0 and 9.0 kb. The hybridization signal intensity is also variable according to the cases. These observations suggest that HBV x gene may be transcribed with flanking cellular sequences and the carcinogenic activity may be diff...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I SaitoY Ohta
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F ImazekiK Koike
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Citations

Jan 17, 2009·Cancer Letters·I Chemin, F Zoulim
Feb 8, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·I Chemin, C Trépo
Oct 31, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J FungM-F Yuen
Jul 13, 2002·Expert Review of Anticancer Therapy·Y ShiratoriM Omata
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Oct 12, 2000·Biochemical and Biophysical Research Communications·W N ChenS W Teng

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