Detection of Hepatitis E Virus in Raw Pork and Pig Viscera As Food in Hebei Province of China

Foodborne Pathogens and Disease
Yansheng GengYouchun Wang

Abstract

Hepatitis E virus (HEV) is an important human pathogen with pigs serving as the main natural animal reservoir. In China, pork is the most popular meat, while pig viscera are also widely consumed. The aim of this study was to evaluate the prevalence of HEV among pigs at slaughter, and assess the presence of HEV in raw pork and pig viscera as food. Samples of pig blood, raw pork, liver, kidney, and blood curd were collected from slaughterhouse or (and) retail market. Anti-HEV antibody in serum samples was detected using enzyme-linked immunosorbent assay based on an ORF2 antigen sandwich kit. HEV RNA was tested by reverse transcription nested polymerase chain reaction (RT-nested PCR) and the viral load was further assessed using quantitative real-time PCR. The final amplicons of RT-nested PCR were sequenced and undergone phylogenetic analysis. Prevalence of antibodies to HEV was 90.4% (104/115) in pigs at slaughtered level, and one serum sample was HEV RNA positive (0.9%, 1/115). HEV RNA was detected in liver, kidney, and blood samples with positivity of 6.1% (7/114), 3.1% (4/129), and 1.2% (2/170) respectively with viral loads ranged 102.4-104.4 (2.4Log-4.4Log) genome equivalents per gram, but not in pork. The HEV RNA prevalence ...Continue Reading

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Citations

Jan 28, 2021·Animals : an Open Access Journal From MDPI·Eleonora ChelliIlaria Di Bartolo
Mar 31, 2021·Foodborne Pathogens and Disease·Gianluigi Ferri, Alberto Vergara
Dec 2, 2020·International Journal of Food Microbiology·Guadalupe Di ColaViviana E Ré

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Datasets Mentioned

BETA
MEGA7.0
MH807312
MH807321
KF691574

Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
PCR
electrophoresis

Software Mentioned

SPSS
Molecular Evolutionary Genetics Analysis , MEGA7

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