Detection of HER2 Amplification in Circulating Tumor Cells of HER2-Negative Gastric Cancer Patients

Targeted Oncology
Yuji MishimaKiyohiko Hatake

Abstract

A key to the successful use of targeted cancer therapy is the ability to preselect patients who are likely to benefit from the treatment according to molecular markers. Assessment for predicting therapy response is mostly done using tumor biopsies. However, these might not truly represent all of the patient's malignant cells because of tumor heterogeneity and/or clonal evolution during disease progression. One potential strategy that can complement primary tumor biopsy is the analysis of circulating tumor cells (CTCs). In this study, we analyzed CTCs of patients with gastric cancer (GC) to find those who were likely to benefit from trastuzumab therapies. We developed an imaging-based method that enabled CTC identification simultaneously with evaluation of HER2 gene amplification (the 3D-IF-FISH method). Then we performed a study enrolling 101 GC patients in whom we analyzed CTCs by both 3D-IF-FISH and an FDA-approved CellSearch system. As compared with the CellSearch system, 3D-IF-FISH methods identified a higher number of patients whose primary tumors were HER2- but who had HER2+ CTCs, suggesting that the 3D-IF-FISH method is effective in preselecting patients for trastuzumab therapies. To demonstrate this, we performed an exp...Continue Reading

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Citations

Mar 19, 2020·Cancers·Phung Thanh HuongHung N Luu
Mar 31, 2018·Oncotarget·Camila Tavares Uchôa GuimarãesDanielle Queiroz Calcagno
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Jun 26, 2020·International Cancer Conference Journal·Suguru HiroseIchinosuke Hyodo
Dec 6, 2018·BioMed Research International·Wen-Chao WangXiao-Feng Zhang
Mar 1, 2021·Cancer Chemotherapy and Pharmacology·Daisuke MatsushitaShoji Natsugoe
Mar 21, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Giandomenico RovielloMartina Catalano

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