PMID: 6540193Jul 1, 1984Paper

Detection of heterogeneity in the chemosensitivity of 9L brain tumor cell lines to 1,3-bis (2-chloroethyl)-1-nitrosourea by the sister chromatid exchange assay

European Journal of Cancer & Clinical Oncology
P J TofilonD F Deen

Abstract

To investigate the potential use of the sister chromatid exchange assay to analyze the heterogeneity of drug response among tumor cell subpopulations, mixtures of 1,3-bis (2-chloroethyl)-1-nitrosourea-sensitive (9L) and -resistant (9L2) rat brain tumor cells were treated in vitro with 2 microM 1,3-bis (2-chloroethyl)-1-nitrosourea. When data were plotted as histograms representing the number of cells vs sister chromatid exchanges/metaphase, two regions corresponding to the 9L and 9L2 populations were obtained. The approximate percentages of 9L and 9L2 in each mixture could be predicted from these histograms. While these results were obtained with a limited model chosen for its simplicity, they suggest that the sister chromatid exchange assay may be useful in the analysis of heterogeneity in drug sensitivity among cell subpopulations in a tumor.

References

Sep 1, 1979·International Journal of Radiation Oncology, Biology, Physics·M M Elkind
Sep 10, 1982·Science·P J TofilonL J Marton
Jul 25, 1959·Nature·H F STICH, H E EMSON

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Citations

Apr 1, 1985·Clinical & Experimental Metastasis·P J TofilonL Milas
Jan 1, 1987·Journal of Cancer Research and Clinical Oncology·W Mueller-Klieser

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