PMID: 6169008Jul 24, 1981Paper

Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA isolation procedure

Nucleic Acids Research
Y GopalakrishnaN Sarkar

Abstract

A new one-step procedure for the isolation of bacterial RNA, involving lysis by proteinase K in the presence of sodium dodecyl sulfate, is described. Pulse-labeled RNA isolated by this procedure for Bacillus brevis, Bacillus subtilis, and Escherichia coli B has been found to contain a substantial fraction (15-40%) of polyadenylated RNA as determined by adsorption to oligo(dT)-cellulose. This contrasts with RNA isolated by procedures involving phenol extraction, a process which appears to lead to the selective loss of polyadenylated RNA. The presence of polyadenylated RNA in E. coli was confirmed by an independent method which involved hybridization with [3H]polyuridylic acid. Using the proteinase K method for RNA isolation, it was possible to demonstrate the in vitro synthesis of polyadenylated RNA by toluene-treated cells of B. brevis, B. subtilis, and E. coli.

References

Jan 30, 1979·Biochemical and Biophysical Research Communications·S Kaur, K Jayaraman
Jan 26, 1978·Biochimica Et Biophysica Acta·N OhtaA Newton
Jul 10, 1975·Nature·H NakazatoM Edmonds
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P R SrinivasanE Rabbani
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jan 1, 1969·Annual Review of Biochemistry·E P Geiduschek, R Haselkorn
Jun 1, 1972·FEBS Letters·U Wiegers, H Hilz
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J E DarnellR J Tushinski
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S Y LeeG Brawerman
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M EdmondsH Nakazato
Mar 13, 1980·Biochemical and Biophysical Research Communications·P Kerjan, J Szulmajster
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M BinaR G Deeley
Feb 1, 1964·Journal of Molecular Biology·B J MCCARTHY, E T BOLTON

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Citations

Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·M A Hayat, D A Mancarella
Feb 18, 1998·Molecular Microbiology·M D JohnsonN Sarkar
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G J Cao, N Sarkar
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G J Cao, N Sarkar
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·E B O'HaraS R Kushner
May 11, 1984·Nucleic Acids Research·J HiscottC Weissmann
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Ciarán Condon
Jan 1, 1997·Annual Review of Biochemistry·N Sarkar
Feb 24, 2011·Wiley Interdisciplinary Reviews. RNA·Bijoy K Mohanty, Sidney R Kushner
Oct 7, 2011·Wiley Interdisciplinary Reviews. RNA·Inês Jesus SilvaCecília Maria Arraiano
Nov 11, 2006·Journal of Microbiological Methods·Ha-Sheng Li-KorotkyWilliam J Doyle
Jul 1, 1983·Archives of Biochemistry and Biophysics·Y Gopalakrishna, N Sarkar
Oct 3, 2018·Wiley Interdisciplinary Reviews. RNA·Nikita VasilyevAlexander Serganov
Aug 13, 2011·Nature Reviews. Microbiology·Marc GüellLuis Serrano
Jan 1, 1986·Journal of Basic Microbiology·R Hanschke, M Hecker
Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kun ZhangChunli Li
Jun 1, 1985·Journal of Bacteriology·J W Brown, J N Reeve
Nov 1, 1983·Journal of Bacteriology·D H CrouchN G Carr
Feb 1, 1990·Journal of Bacteriology·M L Holmes, M L Dyall-Smith
Apr 1, 2007·EcoSal Plus·Sidney R Kushner

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