Detection of Histomonas meleagridis DNA in different organs after natural and experimental infections of meat turkeys

Avian Diseases
Rüdiger HauckHafez M Hafez

Abstract

Histomonas meleagridis infection of turkeys is usually accompanied by a severe disease with unspecific clinical symptoms but with distinct pathological lesions in the ceca and liver. In the literature some macro- and microscopic evidence of the spread of histomonads to the other organs has been provided. The aim of the present investigations was to use real-time polymerase chain reaction (PCR) to demonstrate the dissemination of H. meleagridis DNA to different organs after natural and experimental infection of meat turkeys. Samples from several organs were collected from a meat-turkey flock, which proved to be naturally infected with histomoniasis, and examined for histomonad DNA by real-time PCR. Histomonad DNA was detected in all investigated ceca, livers, spleens, kidneys, and pooled brain swabs. Additionally it was found in 75% of investigated samples from bursae of Fabricius, in 50% of investigated duodenums, and in 40% of investigated jejunum samples. After experimental intracloacal infection of 3-wk-old turkey poults with 147,500 histomonads, similar samples were collected from all turkeys that died. After a 3-wk observation period the surviving birds, as well as the noninfected control group, were euthanatized and sampl...Continue Reading

Citations

Feb 18, 2009·Parasitology Research·Lotfi AbdulRahman, Hafez Mohamed Hafez
Nov 20, 2012·Parasitology Research·Rüdiger Hauck, Hafez M Hafez
Oct 14, 2006·Archives of Animal Nutrition·Hafez Mohamed Hafez, Rüdiger Hauck
Feb 7, 2008·Avian Diseases·Rüdiger Hauck, Hafez Mohamed Hafez
Jan 13, 2015·Veterinary Parasitology·Michael HessPetra Ganas
Sep 29, 2017·Avian Diseases·Steven Clark, Emily Kimminau
Sep 29, 2017·Avian Diseases·Mariana de Mello Zanim MichelazzoSelwyn Arlington Headley
Feb 11, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ana Carolina EwbankCarlos Sacristán

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