PMID: 9551602Apr 29, 1998Paper

Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive patients by simultaneous flow cytometric analysis of intracellular HIV RNA and cellular immunophenotype

Cytometry
B K PattersonC Goolsby

Abstract

Determinations of plasma HIV viral RNA copy numbers help to define the kinetics of HIV-1 infection in vivo and to monitor antiretroviral therapy. However, questions remain regarding the identity of various infected cell types contributing to this free virus pool and to the in vivo lifecycle of HIV during disease progression. Characterization of a novel fluorescence in situ hybridization (FISH) assay employing a pool of labeled oligonucleotide probes directed against HIV RNA was done followed by coupling of the FISH assay with simultaneous surface immunophenotyping to address these questions. In vitro characterizations of this assay using tumor necrosis factor-alpha stimulated and unstimulated ACH-2 cells demonstrated the ability to detect < 5% HIV RNA positive cells with a sensitivity of < 30 RNA copies per cell. Peripheral blood mononuclear cells from 39 HIV-seropositive patients on no, single, combination, or triple drug therapy and 8 HIV-seronegative patients were examined. The majority of HIV-positive patients (24/39) harbored monocytes positive for HIV RNA and a significantly higher fraction of patients with high plasma viral load carried positive monocytes (13/16) than did patients in the low plasma viral load group (11/2...Continue Reading

Citations

Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Oct 22, 2009·Journal of Leukocyte Biology·Woong-Ki KimKenneth Williams
Oct 20, 2012·American Journal of Reproductive Immunology : AJRI·Jackeline Soto-RiveraPhalguni Gupta
Feb 24, 2015·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Alex José Leite TorresCarlos Brites
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·J J McSharry
Oct 24, 2014·Journal of Clinical Microbiology·Amanda CharginBruce K Patterson
Mar 19, 1999·Cytometry·E BednerZ Darzynkiewicz
Jun 26, 2004·Journal of Leukocyte Biology·Simon J PotterNitin K Saksena
Jan 29, 2018·Current HIV/AIDS Reports·Julia NiesslDaniel E Kaufmann
Jan 31, 2018·Retrovirology·Alexander O Pasternak, Ben Berkhout
May 23, 2002·Current Infectious Disease Reports·Claire ChougnetAlan L. Landay
Jun 9, 2007·Bulletin of Mathematical Biology·J GuedjD Commenges
Jan 12, 1999·Nature Medicine·S J BrodieS R Riddell
Aug 29, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J C Martín, J C Bandrés
Jan 9, 2016·Current Protocols in Cytometry·Kah Teong SohPaul K Wallace
Jul 12, 2017·Journal of Dairy Science·Elke DepreesterGeert Opsomer

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